Expressions and activities of cell cycle regulatory molecules during the transition from myocyte hyperplasia to hypertrophy
Expressions and activities of cell cycle regulatory molecules during the transition from myocyte hyperplasia to hypertrophy
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DOI：
10.1006/jmcc.1998.0808
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发表时间：
1998-10-01
影响因子：
5
通讯作者：
Brooks, G
中科院分区：
文献类型：
--
作者：
Poolman, RA;Brooks, G
细胞周期依赖性分子在控制心肌细胞从增生到肥大的转换中的作用尚不清楚，尽管在大鼠中，该过程发生在出生后的第3天和第4天之间。本研究采用荧光激活细胞分选法（fluorescence activated cell sorting，FACS）测定了细胞周期;和（2）通过逆转录酶-聚合酶链反应（RT-PCR）、免疫印迹和体外激酶测定，在从2，3，4和5日龄的动物。在从增生到肥大的过渡过程中，处于细胞周期S期的细胞百分比显著降低（2、3、4和5日龄的细胞分别为5.5%、3.5%、2.3%和1.9%，P
The role of cell cycle dependent molecules in controlling the switch from cardiac myocyte hyperplasia to hypertrophy remains unclear, although in the rat this process occurs between day 3 and 4 after birth. In this study we have determined (1) cell cycle profiles by fluorescence activated cell sorting (FACS); and (2) expressions, co-expressions and activities of a number of cyclins, cyclin-dependent kinases (CDKs) and CDK inhibitors by reverse transcriptase-polymerase chain reaction (RT-PCR), immunoblotting and in vitro kinase assays in freshly isolated rat cardiac myocytes obtained from 2, 3, 4 and 5-day-old animals. The percentage of myocytes found in the S phase of the cell cycle decreased significantly during the transition from hyperplasia to hypertrophy (5.5, 3.5, 2.3 and 1.9% of cells in 2-, 3-, 4- and 5-day-old myocytes, respectively, P