[The application of 4-color fluorescence labeling antibodies and its significance in immunophenotyping for leukemia by flow cytometer].
[The application of 4-color fluorescence labeling antibodies and its significance in immunophenotyping for leukemia by flow cytometer].
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发表时间：
2002-10
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通讯作者：
Yan-rong Liu;Hong-gang Yu;Yan Chang;Shan-shan Chen
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作者：
Yan-rong Liu;Hong-gang Yu;Yan Chang;Shan-shan Chen
为了证明4色荧光标记流式细胞术对白血病免疫分型的意义，我们用4色荧光标记单克隆抗体（mabs）对88例急性白血病患者的骨髓和外周血进行了流式细胞术分析。在4管抗体组合中，用13种不同的单克隆抗体染色可将90%以上的病例分类为急性髓细胞白血病、T淋巴细胞白血病或b淋巴细胞白血病。AML-M(2) -M(7)也可以同时识别。另外一管4个单克隆抗体的组合可用于以上4管未确定的进一步分型。AML-M(0)/M(1)患者在细胞化学中可能是髓过氧化物酶（MPO）阴性，可以通过膜和细胞质抗体标记来识别。总之，四色流式细胞术分析提高了白血病免疫分型的准确性。
To prove the significance of 4-color fluorescence labeling flow cytometric analysis for leukemia immunophenotyping, bone marrow and peripheral blood samples from 88 cases with acute leukemia were analyzed by means of flow cytometry with 4-color fluorescence labeling monoclonal antibodies (McAbs). More than 90% of cases could be classified into acute myeloid, T- or B-lymphocytic leukemia by staining with 13 different kinds of monoclonal antibodies within 4 tubes of combination of antibodies. AML-M(2) - M(7) could also be identified simultaneously. Another one tube of combination of 4 McAbs could be used for further subtyping of those who were undetermined by the above 4 tubes. Patients with AML-M(0)/M(1) which may be negative of myeloperoxidase (MPO) in cytochemistry can be identified by labeling with both membrane and cytoplasmic antibodies. In conclusion, the accuracy of leukemia immunophenotyping has been improved by 4-color flow cytometry analysis.