Phenotypic plasticity of Schwann cells and enteric glial cells in response to the microenvironment.
Phenotypic plasticity of Schwann cells and enteric glial cells in response to the microenvironment.
复制标题
雪旺细胞和肠神经胶质细胞对微环境的表型可塑性。
DOI：
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发表时间：
1991
影响因子：
11.1
通讯作者：
N. L. Le Douarin
中科院分区：
文献类型：
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作者：
C. Dulac;N. L. Le Douarin
我们早些时候生产的单克隆抗体对雪旺细胞髓鞘蛋白（SMP），糖蛋白表达的雪旺细胞（SC），但不是卫星细胞的神经节或肠神经胶质细胞。我们现在研究是否SMP的表达是周围神经系统的不同隔室的环境调节。从胚胎第7天到E11天，来自中-后颅、迷走神经或神经轴干水平的鹌鹑神经嵴细胞与肠壁、皮肤或肌肉组织异时相关。这些嵌合体器官的共培养显示，在正常发育的神经胶质细胞，其特征在于HNK 1免疫反应性和鹌鹑核标记物，表达SMP表型只在皮肤和肌肉，未能这样做，在肠道。然而，当鹌鹑坐骨神经的SMP + SC与鸡肠共培养时，这些细胞迅速失去了它们最初的SMP免疫反应性。相反，当与肌肉和皮肤相关联时，SC保持SMP+，即使在完全不存在神经元细胞的情况下。从E8到E15鹌鹑肠的肠丛在从肠间充质环境中撤出时表达SMP+和层粘连蛋白。这些结果表明，SMP可以由肠神经胶质细胞表达，并且SC SMP表型被肠壁环境强烈抑制。此外，这些结果强烈表明，这两种类型的神经胶质细胞属于同一谱系，其终端表型是通过细胞间的相互作用调制。
We produced earlier a monoclonal antibody against Schwann cell myelin protein (SMP), a glycoprotein expressed on Schwann cells (SC) but not on satellite cells of the ganglia or enteric glial cells. We now studied whether SMP expression is environmentally regulated in the different compartments of the peripheral nervous system. Quail neural-crest cells from either mes-metencephalic, vagal, or truncal levels of the neuraxis were heterochronically associated with gut wall, skin, or muscle tissues from embryonic day (E) 7 to E11 chickens. Coculture of these chimeric organs revealed that as in normal development glial cells, characterized by HNK1 immunoreactivity and the quail nuclear marker, expressed the SMP phenotype exclusively in skin and muscle, failing to do so in gut. However, when SMP+ SC from quail sciatic nerves were cocultured with chicken gut, these cells rapidly lost their initial SMP immunoreactivity. In contrast, when associated with muscle and skin, SC remained SMP+, even in the complete absence of neuronal cells. Enteric plexuses from E8 to E15 quail gut express SMP+ and laminin when withdrawn from the intestinal-mesenchyme environment. These results show that SMP can be expressed by enteric glial cells and that the SC SMP phenotype is strongly inhibited by the gut-wall environment. Moreover, these results strongly suggest that these two types of glial cells belong to the same lineage and that their terminal phenotype is modulated through cell-to-cell interactions.