Translational repression restricts expression of the C-elegans Nanos homolog NOS-2 to the embryonic germline
Translational repression restricts expression of the C-elegans Nanos homolog NOS-2 to the embryonic germline
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DOI：
10.1016/j.ydbio.2005.11.046
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发表时间：
2006-04-01
影响因子：
2.7
通讯作者：
Subramaniam, K
中科院分区：
文献类型：
--
作者：
D'Agostino, I;Merritt, C;Subramaniam, K
nanos基因家族成员是生殖细胞发育的进化保守调节因子。在一些生物体中，Nanos蛋白表达仅限于早期胚胎发生期间的原始生殖细胞（PGCs）。在这里，我们调查的秀丽隐杆线虫nanos同系物NOS-2的调节。我们发现nos-2 RNA受到抑制。在成年生殖系中，nos-2 RNA的翻译在生长的卵母细胞中被抑制，并且这种抑制依赖于nos-2 3 'UTR中的短stein环。在胚胎中，nos-2翻译在早期卵裂球中受到抑制，这种抑制依赖于nos-2 3 'UTR中的第二个区域。nos-2 RNA在体细胞卵裂球中也通过不依赖于翻译抑制的过程降解，并且需要CCCH指蛋白MEX-5和MEX-6。最后，基因质组分POS-1激活PGC中的nos-2翻译。翻译抑制、RNA降解和基因质激活的组合也参与了果蝇和斑马鱼中nanos同系物的调节，表明存在保守机制将nanos表达限制在种系中。(c)2005年爱思唯尔公司All rights reserved.
Members of the nanos gene family are evolutionarily conserved regulators of germ cell development. In several organisms, Nanos protein expression is restricted to the primordial genii cells (PGCs) during early embryogenesis. Here, we investigate the regulation of the Caenorhabditis elegans nanos homolog nos-2. We find that the nos-2 RNA is translationally repressed. In the adult germline, translation of the nos-2 RNA is inhibited in growing oocytes, and this inhibition depends on a short stein loop in the nos-2 3'UTR. In embryos, nos-2 translation is repressed in early blastomeres, and this inhibition depends on a second region in the nos-2 3'UTR. nos-2 RNA is also degraded in somatic blastomeres by a process that is independent of translational repression and requires the CCCH finger proteins MEX-5 and MEX-6. Finally, the genii plasm component POS-1 activates nos-2 translation in the PGCs. A combination of translational repression, RNA degradation, and activation by genii plasm has also been implicated in the regulation of nanos homologs in Drosophila and zebrafish, suggesting the existence of conserved mechanisms to restrict nanos expression to the germline. (c) 2005 Elsevier Inc. All rights reserved.