A Gene Encoding Pentatricopeptide Repeat Protein Partially Restores Fertility in RT98-Type Cytoplasmic Male-Sterile Rice
A Gene Encoding Pentatricopeptide Repeat Protein Partially Restores Fertility in RT98-Type Cytoplasmic Male-Sterile Rice
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DOI：
10.1093/pcp/pcw135
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发表时间：
2016-10-01
影响因子：
4.9
通讯作者：
Toriyama, Kinya
中科院分区：
文献类型：
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作者：
Igarashi, Keisuke;Kazama, Tomohiko;Toriyama, Kinya
水稻细胞质雄性不育系具有野生种的细胞质和栽培稻的核基因组，对研究线粒体基因组与核基因组之间的遗传互作具有重要价值。RT98型不育系RT98A和育性恢复系RT98C携带野生普通野生稻的细胞质和台中65栽培稻的核基因组。基于经典的杂交实验，据报道，育性通过来自细胞质供体的暂定单基因Rf 98的存在而在配子体中恢复。Rf98的精细定位显示，至少有两个基因，这是紧密定位，需要在RT98A完全育性恢复。在这里，我们确定了七个五肽重复序列（PPR）基因，位于一个170 kb的区域作为候选人的Rf 98。互补试验表明，这些PPR基因之一，PPR 762，导致在部分恢复的育性与结实率高达9.3%。因此，PPR 762是RT98型不育系的一个重要育性恢复基因。低结实率表明，Rf98位点附近的一些其他基因也是结实完全恢复所必需的。
Cytoplasmic male sterility (CMS) lines in rice, which have the cytoplasm of a wild species and the nuclear genome of cultivated rice, are of value for the study of genetic interactions between the mitochondrial and nuclear genomes. The RT98-type CMS line RT98A and the fertility restorer line RT98C carry the cytoplasm of the wild species Oryza rufipogon and the nuclear genome of the Taichung 65 cultivar (Oryza sativa L.). Based on a classical crossing experiment, fertility is reported to be restored gametophytically by the presence of a tentative single gene, designated Rf98, which is derived from the cytoplasm donor. Fine mapping of Rf98 revealed that at least two genes, which are closely positioned, are required for complete fertility restoration in RT98A. Here, we identified seven pentatricopeptide repeat (PPR) genes that are located within a 170 kb region as candidates for Rf98. Complementation tests revealed that the introduction of one of these PPR genes, PPR762, resulted in the partial recovery of fertility with a seed setting rate up to 9.3%. We conclude that PPR762 is an essential fertility restorer gene for RT98-type CMS. The low rate of seed setting suggested that some other genes near the Rf98 locus are also necessary for the full recovery of seed setting.