A study of precision in modern liquid Chromatography using a dedicated computer
A study of precision in modern liquid Chromatography using a dedicated computer
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使用专用计算机研究现代液相色谱的精密度
DOI：
10.1016/s0021-9673(00)97046-8
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
B. Karger
中科院分区：
文献类型：
--
作者：
H. Barth;E. Dallmeier;G. Courtois;H. E. Keller;B. Karger
描述了小型数字计算机与液相色谱仪的在线耦合。使用一种新的实时语言 MIRACL II 进行编程。介绍了实验装置并描述了 ndw 自动采样环路阀。该系统已针对商用液相色谱设备可达到的峰面积、峰高和保留的精度进行了研究。发现相对于注射器进样，使用采样阀显着提高了峰面积和高度的精度。还研究了几种泵送系统对精密度的影响，发现峰面积和高度的相对标准偏差存在显着差异。还研究了峰面积的相对标准偏差与样本量的关系。此外，还显示了流动相流速对检测限和精度的重要作用。最后，给出了关于短期和长期保留重现性的一些结果。
The on-line coupling of a small digital computer to a liquid chromatograph is described. A new real-time language, MIRACL II, is used for programming. The experimental set-up is presented and a ndw automatic sample loop valve is described. The system has been studied in terms of the precision in peak area, peak height and retention attainable with commercial liquid chromatographic equipment. Precision in peak area and height is found to be significantly improved with the sampling valve relative to syringe injection. The influence of several pumping systems on precision was also studied and marked differences in the relative standard deviations of peak area and height were found. The relationship of the relative standard deviation of the peak area to sample size has also been studied. In addition, the important role of the flow-rate of the mobile phase on detection limits and precision is shown. Finally, some results are presented on the reproducibility of retention over short and long time periods.