Inflammatory status in patients with chronic renal failure: the role of PTX3 and pro-inflammatory cytokines.
Inflammatory status in patients with chronic renal failure: the role of PTX3 and pro-inflammatory cytokines.
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慢性肾功能衰竭患者的炎症状态：PTX3 和促炎细胞因子的作用。
DOI：
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发表时间：
2007
影响因子：
5.4
通讯作者：
P. Castellino
中科院分区：
文献类型：
--
作者：
G. Malaponte;M. Libra;Y. Bevelacqua;Paola Merito;P. Fatuzzo;F. Rapisarda;M. Cristina;Gabriele Naselli;F. Stivala;M. C. Mazzarino;P. Castellino
在不同的慢性肾功能衰竭（CRF）患者中，已记录了几种急性时相蛋白（如C反应蛋白（CRP））的血浆水平升高。本研究的目的是确定五聚蛋白-3（PTX-3）是否是CRF炎症的可靠标志物。从接受血液透析（HD）前后的43例患者、未接受HD治疗的45例尿毒症患者（UR）和25例健康对照者中采集血浆样本和单核细胞。通过ELISA分析血浆和单核细胞样本中PTX 3、肿瘤坏死因子-α（TNF-α）、白细胞介素-1 β（IL-1 β）和白细胞介素-6（IL-6）的水平; CRF患者中所有这些蛋白水平均高于对照组。HD后，血浆PTX 3和细胞因子水平升高。在HD患者中观察到CRP的个体间和个体内变化，而PTX 3血浆水平稳定。HD前后患者未受刺激的单核细胞释放的PTX 3、TNF-α、IL-1 β和IL-6均高于UR患者和对照组。脂多糖刺激后，HD前患者的所有值均高于UR患者，但低于对照组。相比之下，HD后未观察到变化。在HD和UR患者中观察到血浆PTX 3与纤维蛋白原、TNF-α和IL-1 β之间的显著相关性。总的来说，这些数据表明，PTX 3蛋白可能代表CRF炎症的额外和稳定的标志物。
Increased plasma levels of several acute phase proteins, such as C-reactive protein (CRP), have been documented among different patients with chronic renal failure (CRF). The aim of the present study was to determine whether pentraxin-3 (PTX3) is a reliable marker of inflammation in CRF. Plasma samples and monocytes were taken from 43 patients before and after undergoing haemodialysis (HD), from 45 uraemic patients (UR) without HD treatment and from 25 healthy controls. Plasma and monocyte samples were analyzed by ELISA for levels of PTX3, tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta) and interleukin-6 (IL-6); all of these protein levels were higher in CRF patients with respect to the controls. After HD, plasma PTX3 and cytokine levels increased. Inter- and intra-individual variations in CRP were observed in HD patients, while PTX3 plasma levels were stable. Release of PTX3, TNF-alpha, IL-1beta and IL-6 by unstimulated monocytes from patients, before and after HD, was higher with respect to UR patients and controls. After lipopolysaccharide stimulation, all values were higher in patients before HD than those in UR patients, but lower when compared to those in the controls. In contrast, no changes were observed after HD. A significant correlation among plasma PTX3 versus fibrinogen, TNF-alpha and IL-1beta was observed in HD and UR patients. Collectively, these data suggest that PTX3 protein may represent an additional and stable marker of inflammation in CRF.
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透析膜对细胞因子释放的影响。
DOI：
10.1681/asn.v491711
发表时间：
1994
期刊：
Journal of the American Society of Nephrology : JASN
影响因子：
--
作者：
Zaoui,P;Hakim,RM
通讯作者：
Hakim,RM
影响因子：
4.4
作者：
A. Mackiewicz;T. Speroff;M. Ganapathi;I. Kushner
通讯作者：
A. Mackiewicz;T. Speroff;M. Ganapathi;I. Kushner
影响因子：
19.6
作者：
Chertow, G. M.;Soroko, S. H.;Mehta, R. L.
通讯作者：
Mehta, R. L.
影响因子：
9.3
作者：
E. Macy;T. Hayes;R. Tracy
通讯作者：
E. Macy;T. Hayes;R. Tracy
TSG-14 蛋白与主要急性期蛋白五聚蛋白家族的关系。
DOI：
--
发表时间：
1994
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Lee,GW;Goodman,AR;Lee,TH;Vilcek,J
通讯作者：
Vilcek,J