3D adaptive optics in a light sheet microscope
3D adaptive optics in a light sheet microscope
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DOI：
10.1364/oe.20.013252
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发表时间：
2012-06-04
期刊：
影响因子：
3.8
通讯作者：
Love, Gordon D.
中科院分区：
文献类型：
--
作者：
Bourgenot, Cyril;Saunter, Christopher D.;Love, Gordon D.
我们报道了一种在成像臂中加入自适应光学的单平面照明显微镜(SPIM)。我们展示了样品安装管如何产生像差，并从实验和计算两个方面量化了像差。采用波前无传感器方法对绿色荧光蛋白(GFP)标记的转基因斑马鱼进行成像。我们展示了图像质量的改善，同时记录了3D“z叠层”，并展示了象差是如何来自鱼的不同深度的。(C)2012年美国光学学会
We report on a single plane illumination microscope (SPIM) incorporating adaptive optics in the imaging arm. We show how aberrations can occur from the sample mounting tube and quantify the aberrations both experimentally and computationally. A wavefront sensorless approach was taken to imaging a green fluorescent protein (GFP) labelled transgenic zebrafish. We show improvements in image quality whilst recording a 3D "z-stack" and show how the aberrations come from varying depths in the fish. (C) 2012 Optical Society of America