Immobilized Monolayer Nanoparticles in a Microfluidic Device for Surface Enhanced Raman Scattering Measurement
Immobilized Monolayer Nanoparticles in a Microfluidic Device for Surface Enhanced Raman Scattering Measurement
复制标题
DOI：
10.14326/abe.6.122
复制
发表时间：
2017
影响因子：
1
通讯作者：
Takumi Hase;Soichiro Ishigaki;K. Shibusawa;Sakuya Hamanaka;Yuki Yabuki;Yuki Tamano;K. Tsukada
中科院分区：
文献类型：
--
作者：
Takumi Hase;Soichiro Ishigaki;K. Shibusawa;Sakuya Hamanaka;Yuki Yabuki;Yuki Tamano;K. Tsukada
微培养装置是细胞实验的有力的生物应用工具，特别是因为它们可以调节物理和化学参数，如流速、剪切应力、氧气和培养基中的分子浓度，以模拟体外生理和病理微环境。然而，由于细胞培养是在封闭的空间中进行的，因此必须反复取出培养样品以监测细胞衍生的代谢物和蛋白质，从而维持细胞微环境。我们报道了一种通过在聚二甲基硅氧烷（PDMS）上自组装纳米颗粒单层获得表面增强拉曼散射（Sers）光谱的简单方法，这种方法通常用于高度生物相容的微生物器件。二氧化硅纳米粒子在O2等离子体处理的PDMS膜上稳定为六方密堆积结构，并使用气相沉积涂覆有银以产生Sers板。当这种Sers板被安装在一个微纳米器件中时，即使在长时间的液体浸泡后，纳米颗粒也不会剥离。Sers光谱表现出稳定的Sers产生和增强因子超过1.5 × 106的拉曼信号从罗丹明6 G相比，没有纳米粒子。获得了乳酸和ATP的Sers光谱，并观察到了由于12 C和13 C-乳酸的质量不同而引起的拉曼位移，表明该方法可用于微电子器件中细胞代谢ux的测定。我们还通过在纳米颗粒膜上直接培养小鼠乳腺癌4 T1细胞获得了细胞膜衍生的Sers光谱。聚二甲基硅氧烷具有良好的生物相容性，常用于制造微生物器件。通过将纳米颗粒与O2等离子体处理的PDMS紧密结合，可以长时间测量细胞的化学反应产物或代谢产物，同时保持微环境。我们预计，这种技术可以被利用作为一个有用的工具，用于分析细胞代谢功能和成像细胞分布，而无需染色在各种病理模型中创建的微生物学设备。
Micro uidic devices are powerful bioapplication tools for cellular experiments in particular, as they can regulate physical and chemical parameters such as the ow rate, shear stress, oxygen, and molecular concentrations in a culture medium to mimic physiological and pathological microenvironments in vitro. However, as cell cultures take place in enclosed spaces, culture samples have to be removed repeatedly to monitor cell-derived metabolites and proteins in order to maintain the cellular microenvironment. We report a simple method for obtaining surface enhanced Raman scattering (SERS) spectra through self-assembly of a nanoparticle monolayer on polydimethylsiloxane (PDMS), which is commonly used in highly biocompatible micro uidic devices. Silica nanoparticles were stabilized as a hexagonal close packed structure on an O2 plasma-processed PDMS membrane, and was coated with silver using vapor deposition to create an SERS plate. When this SERS plate was installed in a micro uidic device, the nanoparticles did not peel off even after long-term uid immersion. The SERS spectra exhibited stable SERS generation and an enhancement factor of more than 1.5 × 106 of the Raman signals from rhodamine 6G compared to the signals without nanoparticles. The SERS spectra of lactate and ATP were obtained, and Raman shifts due to the different masses of 12Cand 13C-lactate were observed, suggesting that the proposed method can be applied to determine the cellular metabolic ux in micro uidic devices. We also obtained cell membrane-derived SERS spectra by culturing murine mammary carcinoma 4T1 cells directly on the nanoparticle membrane. PDMS has high biocompatibility and is often used for fabricating micro uidic devices. Through tight binding of the nanoparticles to the O2 plasma-treated PDMS, the chemical reaction products or the metabolites from cells can be measured for a long duration, while maintaining the microenvironment. We anticipate that this technology can be utilized as a useful tool for analyzing cellular metabolic functions and imaging cellular distributions without staining in various pathological models created in micro uidic devices.