RNA-dependent RNA polymerase 1 in potato (Solanum tuberosum) and its relationship to other plant RNA-dependent RNA polymerases.
RNA-dependent RNA polymerase 1 in potato (Solanum tuberosum) and its relationship to other plant RNA-dependent RNA polymerases.
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DOI：
10.1038/srep23082
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发表时间：
2016-03-16
影响因子：
4.6
通讯作者：
Carr JP
中科院分区：
文献类型：
--
作者：
Hunter LJ;Brockington SF;Murphy AM;Pate AE;Gruden K;MacFarlane SA;Palukaitis P;Carr JP
细胞RNA依赖性RNA聚合酶（RDRs）催化双链RNA的合成，所述双链RNA可用于启动或放大RNA沉默。拟南芥有六个RDR基因; RDR 1、2和6在抗病毒RNA沉默中起作用。RDR 6是组成型表达，但RDR 1的表达升高植物处理后的防御性植物激素。RDR 1也有助于基础病毒抗性。RDR 1在包括A. thaliana、烟草（Nicotianatabacum）、N. benthamiana，N. attenuata和番茄（Solanum lycopersicum）中，但在马铃薯（S.块根）。StRDR 1被鉴定并显示出对水杨酸有反应。StRDR 1转录积累下降，在组成型表达发夹结构的转基因马铃薯植物，这些植物的挑战与三种病毒：马铃薯病毒Y，马铃薯病毒X，和烟草花叶病毒。StRDR 1基因表达的抑制并没有增加马铃薯对这些病毒的易感性。对存在于马铃薯和一系列其他植物物种中的RDR基因的系统发育分析鉴定了一个新的RDR基因家族，该家族不存在于马铃薯中，仅在蔷薇科植物中发现（但显然在蔷薇科植物A中丢失）。thaliana），我们建议将其命名为RDR 7。
Cellular RNA-dependent RNA polymerases (RDRs) catalyze synthesis of double-stranded RNAs that can serve to initiate or amplify RNA silencing. Arabidopsis thaliana has six RDR genes; RDRs 1, 2 and 6 have roles in anti-viral RNA silencing. RDR6 is constitutively expressed but RDR1 expression is elevated following plant treatment with defensive phytohormones. RDR1 also contributes to basal virus resistance. RDR1 has been studied in several species including A. thaliana, tobacco (Nicotiana tabacum), N. benthamiana, N. attenuata and tomato (Solanum lycopersicum) but not to our knowledge in potato (S. tuberosum). StRDR1 was identified and shown to be salicylic acid-responsive. StRDR1 transcript accumulation decreased in transgenic potato plants constitutively expressing a hairpin construct and these plants were challenged with three viruses: potato virus Y, potato virus X, and tobacco mosaic virus. Suppression of StRDR1 gene expression did not increase the susceptibility of potato to these viruses. Phylogenetic analysis of RDR genes present in potato and in a range of other plant species identified a new RDR gene family, not present in potato and found only in Rosids (but apparently lost in the Rosid A. thaliana) for which we propose the name RDR7.