Molecular subtyping of Treponema pallidum in an Arizona county with increasing syphilis morbidity:: Use of specimens from ulcers and blood
Molecular subtyping of Treponema pallidum in an Arizona county with increasing syphilis morbidity:: Use of specimens from ulcers and blood
复制标题
DOI：
10.1086/320698
复制
发表时间：
2001-06-01
影响因子：
6.4
通讯作者：
St Louis, ME
中科院分区：
文献类型：
--
作者：
Sutton, MY;Liu, H;St Louis, ME
一种基于分子的梅毒螺旋体分型系统用于调查亚利桑那州马里科帕县梅毒的增加。用梅毒T. pallidum DNA聚合酶I基因聚合酶链反应（PCR）扩增法检测梅毒患者生殖器溃疡或全血标本。阳性标本通过PCR扩增2个可变基因进行分型。总的来说，44份溃疡标本中的41份（93%）和15份血液标本中的4份（27%）产生了可分型的梅毒螺旋体DNA。45例标本中24例（53%）为14f亚型；鉴定出的其他亚型包括4f、4i、5f、12a、12f、14a、14d、14e和14i。仅有2份标本来自流行病学相关的患者。本研究表明，在梅毒高发地区可发现多种梅毒t型，但以1种亚型（14f）为主。4个可分型的标本来自血液，这是一种新发现的用于分型的标本来源。
A molecular-based subtyping system for Treponema pallidum was used during an investigation of increasing syphilis in Maricopa County, Arizona. Genital ulcer or whole blood specimens from patients with syphilis were assayed by a polymerase chain reaction (PCR) amplification of a T. pallidum DNA polymerase I gene. Positive specimens were typed on the basis of PCR amplification of 2 variable genes. In all, 41 (93%) of 44 of ulcer specimens and 4 (27%) of 15 blood specimens yielded typeable T. pallidum DNA. Twenty-four (53%) of 45 specimens were subtype 14f; other subtypes identified included 4f, 4i, 5f, 12a, 12f, 14a, 14d, 14e, and 14i. Only 2 specimens were from epidemiologically linked patients. This investigation demonstrates that multiple subtypes of T. pallidum can be found in an area with high syphilis morbidity, although 1 subtype (14f) was predominant. Four typeable specimens were from blood, a newly identified specimen source for subtyping.