Inhibition of α-glucosidases by tea polyphenols in rat intestinal extract and Caco-2 cells grown on Transwell
Inhibition of α-glucosidases by tea polyphenols in rat intestinal extract and Caco-2 cells grown on Transwell
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DOI：
10.1016/j.foodchem.2021.130047
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发表时间：
2021-05-21
期刊：
影响因子：
8.8
通讯作者：
Oliviero, Teresa
中科院分区：
文献类型：
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作者：
Kan, Lijiao;Capuano, Edoardo;Oliviero, Teresa
以大鼠小肠酶和人Caco-2细胞为研究对象，研究了阿卡波糖、表没食子儿茶素没食子酸酯、表儿茶素没食子酸酯和4种富含多酚的茶叶提取物（白色、绿色、乌龙茶、红茶）对麦芽糖酶、蔗糖酶、异麦芽糖酶和葡萄糖淀粉酶活性的抑制作用。对于大鼠肠道酶混合物，所有四种茶提取物都非常有效地抑制麦芽糖酶和葡萄糖淀粉酶活性，但只有白色茶提取物抑制蔗糖酶和异麦芽糖酶活性，且抑制作用有限。对麦芽糖酶活性有混合型抑制作用。茶提取物与阿卡波糖组合对大鼠麦芽糖酶活性产生协同抑制作用。在Transwell中进行Caco-2细胞实验。绿色茶提取物和表没食子儿茶素没食子酸酯对人蔗糖酶活性有剂量依赖性抑制作用，但对大鼠蔗糖酶活性无抑制作用。在麦芽糖酶活性上观察到相反的情况。结果突出了在两个研究的模型系统中的不同响应，并且表明茶多酚是α-葡萄糖苷酶活性的良好抑制剂。
Inhibition of maltase, sucrase, isomaltase and glucoamylase activity by acarbose, epigallocatechin gallate, epicatechin gallate and four polyphenol-rich tea extract from white, green, oolong, black tea, were investigated by using rat intestinal enzymes and human Caco-2 cells. Regarding rat intestinal enzyme mixture, all four tea extracts were very effective in inhibiting maltase and glucoamylase activity, but only white tea extract inhibited sucrase and isomaltase activity and the inhibition was limited. Mixed-type inhibition on rat maltase activity was observed. Tea extracts in combination with acarbose, produced a synergistic inhibitory effect on rat maltase activity. Caco-2 cells experiments were conducted in Transwells. Green tea extract and epigallocatechin gallate show dose-dependent inhibition on human sucrase activity, but no inhibition on rat sucrase activity. The opposite was observed on maltase activity. The results highlighted the different response in the two investigated model systems and show that tea polyphenols are good inhibitors for alpha-glucosidase activity.