Cryopreservation of human spermatozoa within human or mouse empty zona pellucidae
Cryopreservation of human spermatozoa within human or mouse empty zona pellucidae
复制标题
DOI：
10.1016/s0015-0282(99)00612-3
复制
发表时间：
2000-04-01
影响因子：
6.7
通讯作者：
Lo, HY
中科院分区：
文献类型：
--
作者：
Hsieh, YY;Tsai, HD;Lo, HY
目的：比较不同种属的空透明带（ZP）用于冷冻保存精子。设计：前瞻性研究。设置：医疗中心的IVF单位。患者：无精子症、少弱精子症或正常精子的患者。干预：通过排空卵母细胞或胚胎的细胞质制备人和小鼠ZP。抽空的ZP被注射了来自严重少弱精子症患者和健康生育男性的精子。主要观察指标：根据ZP和精子的不同来源，比较每个ZP的精子数、冷冻前和解冻后的活动精子数、冷冻后每个ZP的丢失精子数和精子回收率。所有组的精子数量、冷冻前和解冻后的活动精子数量、每个ZP的精子丢失数量和精子回收率均相当。冷冻前和解冻后的活动精子总数平均值、解冻后的非活动精子平均值、解冻后的平均丢失精子数和精子回收率分别为14.5%、11.8%、1.0%、1.5%和82%。透明带是冷冻保存睾丸精子提取或显微外科附睾精子抽吸或严重少弱精子症患者精子的理想载体。当人类和小鼠ZP用于精子储存时没有差异。
Objective: To compare the empty zona pellucidae (ZP) of different species for use in the cryopreservation of spermatozoa.Design: Prospective study.Setting: An IVF unit of a medical center.Patient(s): Patients with azoospermia, oligoasthenozoospermia, or normal spermatozoa.Intervention(s): Human and mouse ZP were prepared by evacuating the cytoplasm of oocytes or embryos. The evacuated ZP were injected with spermatozoa from patients with severe oligoasthenozoospermia and from healthy, fertile men. After the freezing and thawing procedure, the spermatozoa were aspirated outside the ZP.Main Outcome Measure(s): The number of spermatozoa per ZP, the number of motile sperm before freezing and after thawing, the number of sperm lost per ZP after freezing, and the sperm recovery rate were compared according to the different origins of the ZP and the sperm.Result(s): The number of spermatozoa, number of motile sperm before freezing and after thawing, number of sperm lost per ZP, and sperm recovery rate were comparable in all groups. The total mean number of motile sperm before freezing and after thawing, the mean number of nonmotile sperm after thawing, the mean number of sperm lost after thawing, and the sperm recovery rate were 14.5%, 11.8%, 1.0%, 1.5%, and 82%, respectively.Conclusion(s): Zona pellucidae are an ideal vehicle for the cryopreservation of sperm collected by testicular sperm extraction or microsurgical epididymal sperm aspiration or from patients with severe oligoasthenozoospermia. There were no differences when human and mouse ZP were used for sperm storage.