Potential for biodegradation of polychlorinated biphenyls (PCBs) by Sinorhizobium meliloti.
Potential for biodegradation of polychlorinated biphenyls (PCBs) by Sinorhizobium meliloti.
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DOI：
10.1016/j.jhazmat.2010.12.008
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发表时间：
2011-02
影响因子：
13.6
通讯作者：
C. Tu;Ying Teng;Yongming Luo;Xiuhua Li;Xianghui Sun;Zhen-gao Li;Wuxing Liu;P. Christie
中科院分区：
文献类型：
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作者：
C. Tu;Ying Teng;Yongming Luo;Xiuhua Li;Xianghui Sun;Zhen-gao Li;Wuxing Liu;P. Christie
采用静息细胞法和土壤微生态系统研究了苜蓿中华根瘤菌对多氯联苯（PCBs）的降解能力及其代谢产物。以2，4，4 ′-TCB为唯一碳源和能量源，在液体培养条件下，可立即观察到生物降解。6d后，2，4，4 ′-TCB的降解率为对照的77.4%。经气相色谱-质谱联用（GC-MS）分析，确定2，4，4 ′-TCB的主要中间体为2-羟基-6-氧代-6-苯基己-2，4-二烯酸（HOPDA）。接种S.苜蓿草可显著促进目标多氯联苯混合物在土壤中的降解。土壤中可培养细菌、真菌和联苯降解菌的数量在接种S.苜蓿草。本研究表明，S.苜蓿草在多氯联苯生物修复中具有良好的应用前景。
Resting cell assay and soil microcosms were set up to investigate the biodegradation capability and metabolic intermediate of polychlorinated biphenyls (PCBs) by a rhizobial strain Sinorhizobium meliloti. Biodegradation was observed immediately after 2,4,4′-TCB was supplied as a sole source of carbon and energy in liquid cultures. After 6 days, the percent biodegradation of 2,4,4′-TCB was 77.4% compared with the control. The main intermediate was identified as 2-hydroxy-6-oxo-6-phenylhex-2,4-dienoic acid (HOPDA) for 2,4,4′-TCB as determined by gas chromatography–mass spectrometry (GC–MS). Inoculation with S. meliloti greatly enhanced the degradation of target PCB mixtures in the soil. Moreover, soil culturable bacteria, fungi and biphenyl degrading bacteria counts showed significant increase after inoculation of S. meliloti. This study suggests that S. meliloti is promising in PCB bioremediation.