Characterization of anti-canine cytokine monoclonal antibodies specific for IFN-gamma: effect of anti-IFN-gamma on renal transplant rejection.
Characterization of anti-canine cytokine monoclonal antibodies specific for IFN-gamma: effect of anti-IFN-gamma on renal transplant rejection.
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IFN-γ特异性抗犬细胞因子单克隆抗体的表征：抗IFN-γ对肾移植排斥的影响。
DOI：
10.1111/j.1399-0039.1994.tb02317.x
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Olson,L
中科院分区：
文献类型：
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作者：
Fuller,L;Carreno,M;Esquenazi,V;Zucker,K;Zheng,S;Roth,D;Burke,G;Nery,J;Asthana,D;Olson,L
在BALB/c小鼠中生成了两种IFN-γ特异性鼠单克隆抗体ADI-1和ADI-23（均为IgG 1 κ）。ADI-1对犬rIFN-γ的亲和力高于对nIFN-γ和人rIFN-γ的亲和力。相比之下，ADI-23对三种IFN-γ制剂显示出相同的亲合力。抗犬IFN-γ mAb不与小鼠和大鼠rIFN-γ结合。还检测了ADI-1和ADI-23 mAb与人rTFN-α的结合，与我们的预期相反，发现ADI-23与人rTFN-α和rIFN-γ的结合显著高于ADI-1。两种抗犬IFN-γ mAb均对48 h PHA诱导的犬成淋巴细胞进行染色。开发了双位点mAb ELISA，其在7-500 ng犬rIFN-γ范围内呈线性，这表明两种mAb检测到犬rIFN-γ分子上的非重叠表位。我们研究了ADI-1对犬同种异体肾移植物延长的影响。通过连续动脉输注ADI‐1治疗的肾移植受体延长至22和25天，而给予IgG 1同种型对照的动物为9和13天。
Two murine monoclonal antibodies specific for IFN‐γ, ADI‐1, and ADI‐23 (both IgG1 kappa), were generated in BALB/c mice. The ADI‐1 exhibited a higher avidity for canine rIFN‐γ than for nIFN‐γ and human rIFN‐γ. In contrast, the ADI‐23 showed equal avidity for the three IFN‐γ preparations. The anti‐canine IFN‐γ mAb did not bind to mouse and rat rIFN‐γ. The ADI‐1, and ADI‐23 mAb were also tested for binding to human rTFN‐α and, contrary to our expectations, it was found that ADI‐23 showed significant binding to human rTFN‐α and rIFN‐γ, in contrast to ADI‐1. Both anti‐canine IFN‐γ mAb stained 48‐h PHA‐induced dog lymphoblasts. A two‐site mAb ELISA was developed, which was linear in the range of 7–500 ng of canine rIFN‐γ, which indicated that the two mAb detected non‐overlapping epitopes on the canine rIFN‐γ molecule. We studied the effect of ADI‐1 on the prolongation of canine renal allografts. Recipients of kidney allografts, that were treated with ADI‐1 by continuous arterial infusion, were prolonged to 22 and 25 days, compared to 9 and 13 days for animals given the IgG1 isotype control.