REQUIREMENT OF TRANSCRIPTION FACTOR PU.1 IN THE DEVELOPMENT OF MULTIPLE HEMATOPOIETIC LINEAGES
REQUIREMENT OF TRANSCRIPTION FACTOR PU.1 IN THE DEVELOPMENT OF MULTIPLE HEMATOPOIETIC LINEAGES
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DOI：
10.1126/science.8079170
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发表时间：
1994-09-09
期刊：
影响因子：
56.9
通讯作者：
SINGH, H
中科院分区：
文献类型：
--
作者：
SCOTT, EW;SIMON, MC;SINGH, H
转录因子PU.1是ets家族的造血特异性成员。通过基因靶向产生在PU.1基因座中携带突变的小鼠。纯合子突变胚胎在妊娠晚期死亡。突变胚胎产生正常数量的巨核细胞和红系祖细胞，但有些表现出红细胞成熟受损。突变的一个不变的后果是在产生B和T淋巴细胞、单核细胞和粒细胞的祖细胞中的多谱系缺陷。因此，淋巴和骨髓谱系的发育程序需要一个共同的遗传功能，可能在多能祖细胞的水平上起作用。
The transcription factor PU.1 is a hematopoietic-specific member of the ets family. Mice carrying a mutation in the PU.1 locus were generated by gene targeting. Homozygous mutant embryos died at a late gestational stage. Mutant embryos produced normal numbers of megakaryocytes and erythroid progenitors, but some showed an impairment of erythroblast maturation. An invariant consequence of the mutation was a multilineage defect in the generation of progenitors for B and T lymphocytes, monocytes, and granulocytes. Thus, the developmental programs of lymphoid and myeloid lineages require a common genetic function likely acting at the level of a multipotential progenitor.