First successful isolation of Entoloma clypeatum species complex from basidiospores
First successful isolation of Entoloma clypeatum species complex from basidiospores
复制标题
首次成功从担子孢子中分离出 Entoloma clypeatum 物种复合体
DOI：
10.1016/j.myc.2019.02.011
复制
发表时间：
2019
期刊：
影响因子：
1.4
通讯作者：
Endo Naoki
中科院分区：
文献类型：
--
作者：
Shishikura Manami;Sugawara Ryo;Takemura Yoshihiro;Sotome Kozue;Maekawa Nitaro;Nakagiri Akira;Endo Naoki
在日本被称为“Harushimeji”的Entoloma clypeatum物种复合体与蔷薇科和榆科植物物种相关。在这项研究中，我们成功地分离出这个真菌群的培养物通过担孢子分离暂定四个Harushimeji种使用改良Norkrans的C（MNC）培养基和MNC培养基含有正丁酸。在25个担子瘤样品中的22个上观察到菌落形成;然而，大多数表现出缓慢和不稳定的生长。分离的菌丝体含有双孢菌丝，并通过真菌核糖体RNA的内转录间隔区的分子系统发育分析进行了鉴定。将显示稳定生长的六个分离物保藏在日本鸟取大学农学院真菌/蘑菇资源和研究中心的真菌培养物保藏中心。这一结果表明，担孢子分离是获得Harushimeji菌株的有用方法。
The Entoloma clypeatum species complex, known as “Harushimeji” in Japan, associates with Rosaceae and Ulmaceae plant species. In this study, we successfully isolated cultures of this fungal group via basidiospore isolation from tentative four Harushimeji species using modified Norkrans's C (MNC) medium and MNC medium containing n-butyric acid. Colony formations were observed on 22 of 25 basidioma samples; however, most exhibited slow and unsteady growth. The isolated mycelia contained dikaryotic hyphae and were identified through molecular phylogenetic analyses of the internal transcribed spacer region of fungal ribosomal RNA. Six isolates showing steady growth were deposited in the fungal culture collection of the Fungus/Mushroom Resource and Research Center, Faculty of Agriculture, Tottori University, Japan. This result indicates that basidiospore isolation is a useful method for obtaining Harushimeji strains.