Green tea catechins prevent cognitive deficits caused by Aβ1-40 in rats
Green tea catechins prevent cognitive deficits caused by Aβ1-40 in rats
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DOI：
10.1016/j.jnutbio.2007.08.008
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发表时间：
2008-09-01
影响因子：
5.6
通讯作者：
Shido, Osamu
中科院分区：
文献类型：
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作者：
Haque, Abdul M.;Hashimoto, Michio;Shido, Osamu
淀粉样β蛋白(Aβ)诱导的氧化应激参与了阿尔茨海默病(AD)的发病机制。相比之下，绿茶儿茶素为大脑神经元提供了强大的抗氧化防御。因此，我们研究了长期服用绿茶钙儿茶素[多酚E(PE)：63%的表儿茶素-3-没食子酸酯，11%的表儿茶素-3-没食子酸酯，6%的(-)-表儿茶素-没食子酸酯]是否能防止AD动物模型的认知障碍，将Aβ(1-40)注入脑室。将5周龄雄性Wistar大鼠随机分为两组：0.0%PE组(单纯饮水)和0.5%PE(5g/L剂量组)。给药20周后，0.0%PE组又分为溶媒组(溶媒组)和Aβ(1~40)组(Aβ组)，而0.5%PE组又分为PE+溶剂组(PE预给药)和PE+Aβ组(PE预给药Aβ组)。使用微型渗透泵将β(1-40)或赋形剂注入脑室。用八安n径向迷宫评估大鼠的行为变化，PE给药26周显著减少Aβ引起的参考和工作记忆错误的数量增加，伴随而来的是海马区脂质过氧化(LPO；40%)和皮质-海马区活性氧(ROS；分别为42%和50%)的减少。PE+赋形剂组大鼠血浆(24%)和海马区(25%)LPO水平以及海马区(23%)和皮质(41%)ROS含量均显著低于赋形剂组。此外，与赋形剂组相比，预先给予PE的大鼠具有更高的血浆铁还原抗氧化能力。我们的结果表明，长期服用绿茶儿茶素可以通过增加抗氧化防御来有效预防Aβ诱导的认知损伤。(C)2008 Elsevier Inc.保留所有权利。
Amyloid beta peptide (A beta)-induced oxidative stress is involved in the pathogenesis of Alzheimer's disease (AD). In contrast, green tea catechins confer potent antioxidative defense to brain neurons. Therefore, we examined whether long-term administration of green tea calechins [Polyphenon E (PE): 63% of epigallocatechin-3-gallate, 11% of epicatechin, 6% of (-)-epigallocatechin and 6% of (-)-epicatechin-gallate] prevents cognitive impairment in an animal model of AD, rats infused with A beta(1-40) into the cerebral ventricle. Five-week-old male Wistar rats fed with in MF diet were randomly divided into two groups: 0.0% PE (rats administered with water only) and 0.5% PE (rats administered with 5 g/L of PE). Twenty weeks after the PE administration, the 0.0% PE group was divided into the Vehicle group) (rats in fused with the solvent used for dissolving A beta) and the A beta(1-40)-infused rat group (A beta group), whereas the 0.5% PE group was divided into the PE+Vehicle group (PE-preadministered vehicle-infused rats) and the PE+A beta group (PE-preadministered A beta-infused rats). A beta(1-40) or vehicle was infused into the cerebral ventricle using a mini osmotic pump. Behavioral changes in the rats were assessed by an eight-ann radial maze, PE administration for 26 weeks significantly decreased the A beta-induced increase in the number of reference and working memory errors, with a concomitant reduction of hippocampal lipid peroxide (LPO; 40%) and cortico-hippocampal reactive oxygen species (ROS; 42% and 50%, respectively). Significantly reduced levels of LPO in the plasma (24%) and hippocampus (25%) as well as those of ROS in the hippocampus (23%) and cortex (41%) were found in the PE+Vehicle group as compared with the Vehicle group. Futhermore, rats with preadministered PE had higher ferric-reducing antioxidation power of plasma as compared with the Vehicle group. Our results suggest that long-term administration of green tea catechins provides effective prophylactic benefits against A beta-induced cognitive impairment by increasing antioxidative defenses. (c) 2008 Elsevier Inc. All rights reserved.