Structure of intracellular teichoic acids from group D streptococci.
Structure of intracellular teichoic acids from group D streptococci.
复制标题
D 组链球菌细胞内磷壁酸的结构。
作者：
A. J. Wicken;J. Baddiley
虽然甘油磷壁酸和核糖醇磷壁酸的分离和结构已经引起了相当大的关注(综述见Baddiley，1962)，但那些发生在细胞下层的那些似乎要广泛得多(Armstrong，Baddiley，Buchanan，Carss&Greenberg，1958；Baddiley&Davison，1961；A.L.Davison，未发表的工作)。为了方便起见，它们被描述为细胞内的磷壁酸，但在迄今研究的两种情况下，它们可能位于细胞壁和原生质膜之间的区域(Hay，Wken&Baddiley，1963)。它们是甘油磷酸聚合物，通常带有糖残留物，并且总是存在D-丙氨酸酯基。尽管在细菌中发现的量相对较少，并且在分离和纯化过程中遇到了相当大的困难，但在乳酸菌中发现的两种结构已经被提出(Keleman&Baddiley，1961；Critchley，Archibald&Baddiley，1962)。磷壁酸的详细化学结构特别令人感兴趣，因为它们的血清学反应(Baddiley&Davison，1961；Haukenes，Ellwood，Baddiley&Oding，1961；Sanderson，Juerens&Strominger，1961)。在链球菌中，D组抗原被Wken，Elliott&Baddiley(1963)鉴定为胞内磷壁酸，他们认为不同生物体D组抗原的血清学行为的差异可能是其化学组成不同的结果。重点研究了粪链球菌Ut8菌株39和链球菌8191菌株(N.C.I.B.)其性质介于屎葡萄球菌和杜氏葡萄球菌之间；这两种微生物都属于血清学群D，胞内磷壁酸是含有葡萄糖的甘油磷酸聚合物。结果表明，39菌株胞内磷壁酸是一种磷酸甘油聚合物，α-糖苷键与甘油连接的是曲二糖[OC-D-葡萄糖-(1-+2)O-D-葡萄糖]，而8191菌株胞内磷壁酸是α-糖苷键连接甘油的曲糖[OC-Dglucopyranosyl-(1-+2)-O-xc-D-glucopyranosyl-(1-+2)O-D-glucose]。两者都含有D-丙氨酸酯残基，与
Although considerable attention has been given to the isolation and structure of glycerol teichoic acid and ribitol teichoic acid from bacterial walls (for review see Baddiley, 1962), those that occur in underlying regions of the cell appear to be much more widespread (Armstrong, Baddiley, Buchanan, Carss & Greenberg, 1958; Baddiley & Davison, 1961; A. L. Davison, unpublished work). They have been described for convenience as 'intracellular' teichoic acids, but in the two cases so far studied it is shown that they are probably located in the region between the wall and the protoplast membrane (Hay, Wicken & Baddiley, 1963). They are glycerol phosphate polymers, usually bearing sugar residues, and D-alanine ester groups are always present. Despite the relatively small amounts found in bacteria, and the considerable difficulties encountered during their isolation and purification, structures have been proposed for two found in lactobacilli (Kelemen & Baddiley, 1961; Critchley, Archibald & Baddiley, 1962). The detailed chemical structure of teichoic acids is of particular interest because of their serological reactions (Baddiley & Davison, 1961; Haukenes, Ellwood, Baddiley & Oeding, 1961; Sanderson, Juergens & Strominger, 1961). In streptococci the group D antigen has been identified as the intracellular teichoic acid by Wicken, Elliott & Baddiley (1963), who suggested that differences in the serological behaviour ofgroup D antigens from different strains of organism might be a consequence of differences in their chemical composition. Particular attention was given to Streptococcus faecaUt8 strain 39 and a Streptococcus strain 8191 (N.C.I.B.) which is intermediate in character between S.faecium and S. durans; both organisms are in serological group D and the intracellular teichoic acids are glycerol phosphate polymers containing glucose. The present work shows that the intracellular teichoic acid from strain 39 is a glycerol phosphate polymer with kojibiose [oc-D-glucopyranosyl-(1-+2)O-D-glucose] in a-glycosidic linkage to glycerol, whereas that from strain 8191 has kojitriose [OC-Dglucopyranosyl-(1-+2)-O-xc-D-glucopyranosyl-(1-+2)O-D-glucose] in a-glycosidic linkage to glycerol. Both possess D-alanine ester residues which, unlike