MECHANOCHEMICAL COUPLING IN FLAGELLA .3. EFFECTS OF SOME UNCOUPLING AGENTS ON PROPERTIES OF FLAGELLAR ATPASE
MECHANOCHEMICAL COUPLING IN FLAGELLA .3. EFFECTS OF SOME UNCOUPLING AGENTS ON PROPERTIES OF FLAGELLAR ATPASE
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DOI：
10.1016/0003-9861(71)90262-1
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发表时间：
1971-01-01
影响因子：
3.9
通讯作者：
BENEDICT, B
中科院分区：
文献类型：
--
作者：
BROKAW, CJ;BENEDICT, B
海胆精子鞭毛经分离和甘油化处理后，是不运动的，但其ATP脱磷酸化速率是运动精子ATP脱磷酸化速率的1.5 ~ 1.2。添加O. 3 M硫脲加到ATP酶测定液中，用O. 6 m KCl和其他几种抑制甘油精子运动的处理，使ATP去磷酸化率增加100%或更多。这显然代表了ATP去磷酸化与产生运动的系统的其他组分的解偶联。解偶联的ATP酶具有较高的Km值，对Mg 2+离子活化的特异性更强，除活性增加外，其它特性也发生了改变。虽然解偶联和提取通常一起发生，但在某些条件下，ATP酶活性可以以较少修饰的形式提取，其仍然被硫脲激活。需要进一步开展工作，以完全控制脱钩过程并了解其影响。
Sea urchin sperm flagella, after isolation and glycerination, are nonmotile, but dephosphorylate ATP at a rate which is 1 5 to 1 2 of the probable rate of ATP dephosphorylation in actively motile spermatozoa. Addition of O. 3 m thiourea to the ATPase assay solutions, extraction with O. 6 m KCl, and several other treatments which inhibit the motility of glycerinated spermatozoa, increase the rate of ATP dephosphorylation by 100% or more. This apparently represents an uncoupling of ATP dephosphorylation from other components of the system which generates movement. The uncoupled ATPase has a higher K m, greater specificity for Mg 2+ ion activation, and other altered characteristics in addition to increased activity. Although uncoupling and extraction usually occur together, under some conditions the ATPase activity can be extracted in a less modified form, which is still activated by thiourea. Further work is required to gain complete control over the uncoupling process and understand its implications.