Differential gene expression in skeletal muscle cells after membrane depolarization
Differential gene expression in skeletal muscle cells after membrane depolarization
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DOI：
10.1002/jcp.20902
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发表时间：
2007-03-01
影响因子：
5.6
通讯作者：
Riveros, Nora
中科院分区：
文献类型：
--
作者：
Juretic, Nevenka;Urzua, Ulises;Riveros, Nora
骨骼肌是一种高度可塑性的组织，具有适应收缩活动所带来的挑战的非凡能力。包括肥大和氧化机制激活在内的适应性反应与特定基因转录活性的瞬时变化有关。为了定义由去极化刺激调控的基因组，我们使用了22 K小鼠寡核苷酸微阵列。在高K+刺激后2、4、18和24 h从C2C12肌管获得总RNA。在微阵列杂交之前，用花青3或5染料标记来自对照和去极化样品的cDNA。黄土标准化，然后统计分析，导致423差异表达的基因，使用未经调整的P值
Skeletal muscle is a highly plastic tissue with a remarkable capacity to adapt itself to challenges imposed by contractile activity. Adaptive response, that include hypertrophy and activation of oxidative mechanisins have been associated with transient changes in transcriptional activity of specific genes. To define the set of genes regulated by a depolarizing stimulus, we used 22 K mouse oligonucleotide microarrays. Total RNA from C2C12 myotubes was obtained at 2, 4, 18, and 24 h after high K+ stimulation. cDNA from control and depolarized samples was labeled with cyanine 3 or 5 dyes prior to microarray hybridization. Loess normalization followed by statistical analysis resulted in 423 differentially expressed genes using an unadjusted P-value