Proteoglycans synthesized by canine intervertebral disc cells grown in a type I collagen-glycosaminoglycan matrix
Proteoglycans synthesized by canine intervertebral disc cells grown in a type I collagen-glycosaminoglycan matrix
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DOI：
10.1089/107632702320934137
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发表时间：
2002-12-01
影响因子：
--
通讯作者：
Spector, M
中科院分区：
文献类型：
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作者：
Rong, Y;Sugumaran, G;Spector, M
本研究的目的是确定犬纤维环细胞合成的蛋白多糖的特性，所述细胞在单层培养物中扩增至第4代，随后在I型胶原-糖胺聚糖基质中生长以用于组织工程。通过凝胶色谱法分析新合成的[S-35]硫酸盐标记的蛋白聚糖，包括用酶和亚硝酸连续消化。培养1周后，在I型胶原-糖胺聚糖基质中合成的细胞相关聚集蛋白聚糖的百分比为52%，而单层细胞为38%。添加外源性透明质酸后，各组中聚集蛋白多糖的百分比仅略有增加，但彼此之间仍存在显著差异。在胶原-糖胺聚糖基质和单层中至少有三种不同的流体动力学大小的蛋白聚糖;胶原基质中的平均大小较大，糖胺聚糖链较长。蛋白聚糖含有硫酸软骨素、硫酸皮肤素、硫酸乙酰肝素和硫酸角质素。本研究结果为胶原-糖胺聚糖基质用于椎间盘组织工程的进一步研究奠定了基础。
The objective, of this study was to determine the characteristics of proteoglycans synthesized by canine annulus fibrosus cells expanded in number in monolayer culture through passage 4 and subsequently grown in a type I collagen-glycosaminoglycan matrix to be employed for tissue engineering. Newly synthesized [S-35]sulfate-labeled proteoglycans were analyzed by gel chromatography, including sequential digestion with enzymes and nitrous acid. After 1 week in culture, the percentage of cell-associated,, aggregated proteoglycans synthesized in type I collagen-glycosaminoglycan matrices was 52% compared with 38% by the cells in monolayer. The percentage of aggregated proteoglycan in each group increased only slightly with the addition of exogenous hyaluronic acid, but remained significantly different from each other. There were at least three different hydrodynamic sizes of proteoglycans both in the collagen-glycosaminoglycan matrix and in monolayer; the average size was larger in the collagen matrices and the glycosaminoglycan chains were longer. The proteoglycans contained chondroitin sulfate, dermatan sulfate, heparan sulfate, and keratan sulfate. The results provide a foundation for future investigations of collagen-glycosaminoglycan matrices for intervertebral disc tissue engineering.