CELL-GROWTH AND ANTI-MICROBIAL ACTIVITY OF MOUSE PERITONEAL-MACROPHAGES IN RESPONSE TO GLUCOCORTICOIDS, CHOLERAGEN AND LIPOPOLYSACCHARIDE
CELL-GROWTH AND ANTI-MICROBIAL ACTIVITY OF MOUSE PERITONEAL-MACROPHAGES IN RESPONSE TO GLUCOCORTICOIDS, CHOLERAGEN AND LIPOPOLYSACCHARIDE
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DOI：
10.1111/j.1348-0421.1980.tb02924.x
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发表时间：
1980-01-01
影响因子：
2.6
通讯作者：
YOKOTA, T
中科院分区：
文献类型：
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作者：
NOZAWA, RT;YANAKI, N;YOKOTA, T
正常的、巯基乙酸刺激的和卡介苗激活的小鼠腹腔巨噬细胞在小鼠L - 929细胞的条件培养基中体外培养。巯基乙酸酯和BCG巨噬细胞增殖迅速，而正常巨噬细胞生长较慢。三种类型的巨噬细胞在培养中有明显的形态学差异。糖皮质激素在药理学浓度下抑制巨噬细胞的生长。其他类固醇，孕酮、己烯雌酚和睾酮的生长抑制作用要低得多。与未处理的细胞相比，10‐6M地塞米松培养的巨噬细胞对假丝酵母菌的抗菌作用降低。胆碱原对巨噬细胞的作用与糖皮质激素相同。毒素在低至10 pg/ml的浓度下抑制生长，每ml 1 ng的霍乱原处理的细胞抗真菌活性降低。同样，10 ng/ml大肠杆菌多糖抑制巯基酸巨噬细胞的生长。然而，与脂多糖孵育的巨噬细胞具有增强的抗念珠菌活性。因此，免疫抑制剂糖皮质激素和胆碱原抑制了巨噬细胞数量的增加和吞噬细胞的杀微生物活性。脂多糖，一种免疫刺激剂，刺激巨噬细胞的活性，但对细胞生长是有毒的。
Normal, thioglycollate‐stimulated and BCG‐activated mouse peritoneal macrophages were cultivatedin vitrowith the conditioned medium of mouse L‐929 cells. The thioglycollate‐ and BCG‐macrophages rapidly proliferated, whereas normal macrophages grew more slowly. A clear morphological difference between the three types of macrophages in the culture was observed. Glucocorticoids inhibited the growth of the macrophages at pharmacological concentrations. Other steroids, progesterone, diethylstilbesterol and testosterone in that order, had a far lower growth‐inhibiting effect. Macrophages cultured with 10‐6M dexamethasone had a reduced antimicrobial effect onCandida parapsilosiscompared with that of the untreated cells. Choleragen had the same effect on the macrophages as glucocorticoids. The toxin inhibited growth at a concentration as low as 10 pg/ml and cells treated with 1 ng of choleragen per ml had decreased antifungal activity. Similarly,Escherichia colilipopolysaccharide at 10 ng/ml inhibited the growth of thioglycollate‐macrophages. However, macrophages incubated with the lipopolysaccharide had enhanced anticandida activity. Thus, the immunosuppressors glucocorticoid and choleragen inhibited both the increase in the number of macrophages and the microbicidal activity of the phagocytes. Lipopolysaccharide, an immunostimulant, stimulated macrophage activity, but was toxic for cell growth.