Single Amino Acid Switch between a Flavin-Dependent Dehalogenase and Nitroreductase.
Single Amino Acid Switch between a Flavin-Dependent Dehalogenase and Nitroreductase.
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DOI：
10.1021/jacs.5b07540
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发表时间：
2015-12-16
影响因子：
15
通讯作者：
Rokita SE
中科院分区：
文献类型：
--
作者：
Mukherjee A;Rokita SE
黄素蛋白内的单个突变能够将脱卤酶的催化活性转换为硝基还原酶。这种功能变化与氧化还原循环过程中硝基-FMN还原酶超家族中差异表达的单电子还原黄素半醌的不稳定相关。因此，在这样一个超家族内的功能多样性有可能从快速进化中产生，其成员应该为开发具有改变的选择特异性的新催化剂提供方便的基础。
A single mutation within a flavoprotein is capable of switching the catalytic activity of a dehalogenase into a nitroreductase. This change in function correlates with a destabilization of the one-electron-reduced flavin semiquinone that is differentially expressed in the nitro-FMN reductase superfamily during redox cycling. The diversity of function within such a superfamily therefore has the potential to arise from rapid evolution, and its members should provide a convenient basis for developing new catalysts with an altered specificity of choice.