CELL-AUTONOMOUS ROLE OF NOTCH, AN EPIDERMAL GROWTH-FACTOR HOMOLOG, IN SENSORY ORGAN DIFFERENTIATION IN DROSOPHILA
CELL-AUTONOMOUS ROLE OF NOTCH, AN EPIDERMAL GROWTH-FACTOR HOMOLOG, IN SENSORY ORGAN DIFFERENTIATION IN DROSOPHILA
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DOI：
10.1073/pnas.88.2.632
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发表时间：
1991-01-01
影响因子：
11.1
通讯作者：
GARCIABELLIDO, A
中科院分区：
文献类型：
--
作者：
DECELIS, JF;MARIBEFFA, M;GARCIABELLIDO, A
Notch (N)基因编码一种跨膜蛋白，其胞外结构域与表皮生长因子同源，胞内结构域可能参与信号转导。在中枢和周围神经系统中，N空等位基因可使大多数表皮细胞转化为神经母细胞。同样的等位基因，被称为Abruptex (Ax)，定位于N蛋白的细胞外区域，导致成人感觉器官的缺失。在成年感觉器官形成的最后阶段进行的镶嵌分析中，这两种类型的等位基因都表现出细胞的自主性。表型不同：缺乏N基因产物的细胞早期分化为感觉器官母细胞，后期分化为神经亚系，而纯合子Ax条件下表皮细胞不进入感觉器官母细胞途径。结果表明，N基因产物在细胞内部发挥作用，可能作为细胞间信号的受体参与感觉器官母细胞的单一性及其子细胞的命运规范。在这个信号转导过程中，Ax突变表现为N+函数的过量。被这些突变修饰的N蛋白作为组成激活。
The gene Notch (N) codes for a transmembrane protein with an extracellular domain that has homologies to epidermal growth factors and an intracellular domain that could be involved in signal transduction. N null alleles cause the transformation of most epidermal cells into neuroblasts in central and peripheral nervous systems. Alleles of the same gene, called Abruptex (Ax), that map to the extracellular domain of N protein cause the absence of adult sensory organs. Both types of alleles show cell autonomy in mosaic analysis carried out in the last stages of the formation of adult sensory organs. The phenotypes are different: cells lacking N gene products differentiate as sensory organ mother cells early and as its neural sublineage later, whereas in the homozygous Ax condition epidermal cells do not enter the sensory organ mother cell pathway. The results indicate that N gene products act internally in the cell, probably as receptors of intercellular signals both in sensory organ mother cell singularization and in fate specification of its daughter cells. Ax mutations behave as an excess of N+ function in this signal transduction process. N proteins modified by these mutations act as constitutively activated.