Demyelination Induced in Aggregating Brain Cell Cultures by a Monoclonal Antibody Against Myelin/Oligodendrocyte Glycoprotein
Demyelination Induced in Aggregating Brain Cell Cultures by a Monoclonal Antibody Against Myelin/Oligodendrocyte Glycoprotein
复制标题
抗髓磷脂/少突胶质细胞糖蛋白单克隆抗体在聚集脑细胞培养物中诱导脱髓鞘
DOI：
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发表时间：
1990
影响因子：
4.7
通讯作者：
J. Matthieu
中科院分区：
文献类型：
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作者：
N. Rosbo;P. Honegger;H. Lassmann;J. Matthieu
针对髓磷脂/少突胶质细胞糖蛋白 (MOG) 的单克隆抗体 (8-18C5) 在聚集的脑细胞培养物中诱导脱髓鞘。随着补体存在下抗 MOG 抗体剂量的增加，髓磷脂碱性蛋白 (MBP) 浓度以剂量相关的方式下降。对 2',3'-环核苷酸 3'-磷酸水解酶的比活性也观察到类似的影响，尽管不太明显。在没有补体的情况下，抗 MOG 抗体不会诱导可检测到的脱髓鞘。与抗 MOG 抗体的效果相反，并且如预期的那样，在补体存在的情况下，抗 MBP 抗体不会使聚集的脑细胞培养物脱髓鞘。这些结果进一步支持了以下观点：MOG（一种位于髓磷脂膜外侧的少量髓磷脂成分）是抗体诱导脱髓鞘的良好靶抗原。事实上，他们表明，针对糖蛋白上单个表位的纯化抗 MOG 抗体不仅可以在体内（如之前所示）产生脱髓鞘，而且可以在培养物中产生脱髓鞘。针对纯化髓磷脂蛋白（如 MBP 和蛋白脂质蛋白）（髓磷脂膜的主要蛋白质成分或髓磷脂相关糖蛋白）的多克隆抗血清尚未进行过这样的观察。这些观察结果可能对抗 MOG 抗体在脱髓鞘疾病中的可能作用具有重要意义。
A monoclonal antibody (8–18C5) directed against myelin/oligodendrocyte glycoprotein (MOG) induced demyelination in aggregating brain cell cultures. With increasing doses of anti‐MOG antibody in the presence of complement, myelin basic protein (MBP) concentrations decreased in a dose‐related manner. A similar, albeit less pronounced, effect was observed on specific activity of 2′,3′‐cyclic nucleotide 3′‐phosphohydrolase. In the absence of complement, anti‐MOG antibody did not induce detectable demyelination. In contrast to the effect of anti‐MOG antibody and as expected, anti‐MBP antibody did not demyelinate aggregating brain cell cultures in the presence of complement. These results provide additional support to the suggestion that MOG, a quantitatively minor myelin component located on the external side of the myelin membrane, is a good target antigen for antibody‐induced demyelination. Indeed, they show that a purified anti‐MOG antibody directed against a single epitope on the glycoprotein can produce demyelination, not only in vivo as previously shown, but also in cultures. Such an observation has not been made with polyclonal antisera raised against purified myelin proteins like MBP and proteolipid protein, the major protein components of the myelin membrane, or myelin‐associated glycoprotein. These observations may have important implications regarding the possible role of anti‐MOG antibodies in demyelinating diseases.
体外脱髓鞘研究：补体系统膜攻击成分的需要。
DOI：
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发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Liu,WT;Vanguri,P;Shin,ML
通讯作者：
Shin,ML