Cellulose-degrading bacteria associated with the invasive woodwasp Sirex noctilio
Cellulose-degrading bacteria associated with the invasive woodwasp Sirex noctilio
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DOI：
10.1038/ismej.2011.14
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发表时间：
2011-08-01
期刊：
影响因子：
11
通讯作者：
Raffa, Kenneth F.
中科院分区：
文献类型：
--
作者：
Adams, Aaron S.;Jordan, Michelle S.;Raffa, Kenneth F.
夜莺是一种入侵的食木黄蜂，威胁着世界上的商业和天然松林。这种昆虫成功地在树上定居取决于寄主防御能力的下降和利用木质基质作为能量来源的能力。我们利用生长试验、细菌关联频率的培养依赖和独立分析以及全基因组分析，探索了其与细菌共生体的潜在关联，这些共生体可能有助于通过植物生物量解构获取营养。我们鉴定出链霉菌（Streptomyces）和γ -变形菌（gamma-Proteobacteria）分别与94%和88%的黄蜂相关。分离链霉菌在所有三种测试的纤维素基质上生长，并在pH 5.6至9的范围内生长。在全基因组测序的基础上，三个链霉菌分离物具有一些最高比例的基因，预测编码碳水化合物活性酶（CAZyme）的序列放线菌。γ -变形杆菌在纤维素衍生物和结构多样的底物氨纤维爆炸处理的玉米秸秆上生长，但不能在微晶纤维素上生长。对Pantoea分离物的基因组分析发现了推测编码CAZymes的基因，大多数预测对半纤维素和更多的单糖有活性。我们认为，包括上述细菌和真菌共生体areolatum Amylostereum在内的一组微生物在降解木质基质方面具有互补功能，这种降解可能有助于S. noctilio获取营养物质，从而有助于其在全球森林栖息地建立的能力。ISME学报（2011）5,1323-1331；doi: 10.1038 / ismej.2011.14;2011年3月3日在线发布
Sirex noctilio is an invasive wood-feeding wasp that threatens the world's commercial and natural pine forests. Successful tree colonization by this insect is contingent on the decline of host defenses and the ability to utilize the woody substrate as a source of energy. We explored its potential association with bacterial symbionts that may assist in nutrient acquisition via plant biomass deconstruction using growth assays, culture-dependent and -independent analysis of bacterial frequency of association and whole-genome analysis. We identified Streptomyces and gamma-Proteobacteria that were each associated with 94% and 88% of wasps, respectively. Streptomyces isolates grew on all three cellulose substrates tested and across a range of pH 5.6 to 9. On the basis of whole-genome sequencing, three Streptomyces isolates have some of the highest proportions of genes predicted to encode for carbohydrate-active enzymes (CAZyme) of sequenced Actinobacteria. gamma-Proteobacteria isolates grew on a cellulose derivative and a structurally diverse substrate, ammonia fiber explosion-treated corn stover, but not on microcrystalline cellulose. Analysis of the genome of a Pantoea isolate detected genes putatively encoding for CAZymes, the majority predicted to be active on hemicellulose and more simple sugars. We propose that a consortium of microorganisms, including the described bacteria and the fungal symbiont Amylostereum areolatum, has complementary functions for degrading woody substrates and that such degradation may assist in nutrient acquisition by S. noctilio, thus contributing to its ability to be established in forested habitats worldwide. The ISME Journal (2011) 5, 1323-1331; doi:10.1038/ismej.2011.14; published online 3 March 2011