Brain-Wide Mapping of Afferent Inputs to Accumbens Nucleus Core Subdomains and Accumbens Nucleus Subnuclei
Brain-Wide Mapping of Afferent Inputs to Accumbens Nucleus Core Subdomains and Accumbens Nucleus Subnuclei
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伏隔核核心子域和伏隔核亚核的传入输入的全脑映射
DOI：
10.3389/fnsys.2020.00015
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发表时间：
2020-03-18
影响因子：
3
通讯作者：
Han, Yunyun
中科院分区：
文献类型：
--
作者：
Ma, Liping;Chen, Wenqi;Han, Yunyun
纹状体的腹侧部分是认知、情绪和行动之间的界面。它由三个主要的亚核组成：即，NAc核心（NAcC）、外侧壳（NAcLS）和内侧壳（NAcMS），表现出功能异质性。因此，确定NAc亚区的突触输入对于理解参与调节不同功能的电路机制是重要的。在这里，我们同时标记的NAc与霍乱毒素亚基B与荧光标记的亚区，然后成像的全脑连续切片与全自动载玻片扫描系统。使用交互式WholeBrain框架，我们表征了对NAcC子域的全脑输入，包括吻侧、尾侧、背侧和腹侧子域（即，rNAcC、cNAcC、dNAcC和vNAcC）和NAc亚核。我们发现不同的脑区，分布从大脑到脑干，投射到NAc。在投射到NAcC的57个脑区中，前嗅核（AON）表现出最大的输入。rNAcC和cNAcC的输入神经元是两个不同的群体，但在相同的上游脑区域上具有相似的分布，而dNAcC和vNAcC的输入神经元在相同的上游区域上表现出略微不同的分布。在投射到NAcLS的55个脑区中，梨状区贡献了大部分输入。在投射到NAcMS的72个脑区中，外侧隔核贡献了大部分的输入。NAcC和NAcLS的输入神经元具有相似的分布，而NAcMS表现出全脑的不同分布。因此，NAcC亚结构域似乎共享相同的上游脑区，尽管具有不同的输入神经元群体和输入比例的轻微差异，而NAcMS亚核从多个上游脑区接收不同的输入。这些结果为进一步了解NAcC亚区和NAcC亚核的不同功能奠定了解剖学基础。
The nucleus accumbens (NAc) is the ventral part of the striatum and the interface between cognition, emotion, and action. It is composed of three major subnuclei: i.e., NAc core (NAcC), lateral shell (NAcLS), and medial shell (NAcMS), which exhibit functional heterogeneity. Thus, determining the synaptic inputs of the subregions of the NAc is important for understanding the circuit mechanisms involved in regulating different functions. Here, we simultaneously labeled subregions of the NAc with cholera toxin subunit B conjugated with multicolor Alexa Fluor, then imaged serial sections of the whole brain with a fully automated slide scanning system. Using the interactive WholeBrain framework, we characterized brain-wide inputs to the NAcC subdomains, including the rostral, caudal, dorsal, and ventral subdomains (i.e., rNAcC, cNAcC, dNAcC, and vNAcC, respectively) and the NAc subnuclei. We found diverse brain regions, distributed from the cerebrum to brain stem, projecting to the NAc. Of the 57 brain regions projecting to the NAcC, the anterior olfactory nucleus (AON) exhibited the greatest inputs. The input neurons of rNAcC and cNAcC are two distinct populations but share similar distribution over the same upstream brain regions, whereas the input neurons of dNAcC and vNAcC exhibit slightly different distributions over the same upstream regions. Of the 55 brain regions projecting to the NAcLS, the piriform area contributed most of the inputs. Of the 72 brain regions projecting to the NAcMS, the lateral septal nucleus contributed most of the inputs. The input neurons of NAcC and NAcLS share similar distributions, whereas the NAcMS exhibited brain-wide distinct distribution. Thus, the NAcC subdomains appeared to share the same upstream brain regions, although with distinct input neuron populations and slight differences in the input proportions, whereas the NAcMS subnuclei received distinct inputs from multiple upstream brain regions. These results lay an anatomical foundation for understanding the different functions of NAcC subdomains and NAc subnuclei.