The transcription factor NF-κB in the demosponge Amphimedon queenslandica:: insights on the evolutionary origin of the Rel homology domain
The transcription factor NF-κB in the demosponge Amphimedon queenslandica:: insights on the evolutionary origin of the Rel homology domain
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DOI：
10.1007/s00427-007-0197-5
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发表时间：
2008-01-01
影响因子：
2.4
通讯作者：
Degnan, Bernard M.
中科院分区：
文献类型：
--
作者：
Gauthier, Marie;Degnan, Bernard M.
Rel/核因子-kappaB(NF-kappa B)和活化T细胞核因子(NFAT)转录因子通过利用其Rel同源结构域(RHD)高特异性地结合DNA来调节一系列生物学过程。最近，有研究表明，这些基因家族的成员存在于矢状珊瑚虫的基因组中，这表明它们早于最新的祖先进化到现存的双边动物。通过在昆士兰Amphimedon queenslandica的基因组中鉴定一个单一的NF-kappa B基因，我们在这里证明了Rel/NF-kappa B家族起源于后生动物的黎明。在真菌和脊椎动物基因组中没有RHD的证据，这支持了RHD是后生动物特有的创新的观点。A.queenslandica基因(AmqNF-kappa B)编码的蛋白质在结构上与脊椎动物的NF-kappa B p50/p52蛋白高度相似，同时具有RHD和Ankyrin(ANK)重复序列。完整的AmqNF-kappa B与缺乏ANK重复的N载体NF-kappa B形成对比，表明原始后生动物的NF-kappa B与当代脊椎动物和海绵形式的配置相同。AmqNF-kappa B在昆士兰红曲霉胚胎发生过程中表达，提示其在胚胎发育中起重要作用。
The Rel/nuclear factor-kappa B (NF-kappa B) and nuclear factor of activated T-cells (NFAT) transcription factors contribute to the regulation of an assortment of biological processes by binding DNA with high specificity using their Rel homology domain (RHD). Recently, it has been shown that members of these gene families are present in the genome of the anthozoan cnidarian Nematostella vectensis, indicating that they predate the evolution of the most recent ancestor to living bilaterians. By identifying a single NF-kappa B gene in the genome of the demosponge Amphimedon queenslandica, a representative of an even earlier branching metazoan lineage, we demonstrate here that the Rel/NF-kappa B family originated at the dawn of the Metazoa. There is no evidence of RHDs in fungal and choanoflagellate genomes, supporting the notion that the RHD is a metazoan-specific innovation. The A. queenslandica gene (AmqNF-kappa B) encodes a protein that is highly similar in structure to the vertebrate NF-kappa B p50/p52 proteins, possessing both a RHD and ankyrin (ANK) repeats. The intact AmqNF-kappa B contrasts with the N. vectensis NF-kappa B, which lacks ANK repeats, and suggests that the ancestral metazoan NF-kappa B was configured identically to contemporary vertebrate and sponge forms. AmqNF-kappa B is expressed during A. queenslandica embryogenesis, suggesting a developmental role.