Uncovering the dynamic evolution of nucleotide-binding site-leucine-rich repeat (NBS-LRR) genes in Brassicaceae
Uncovering the dynamic evolution of nucleotide-binding site-leucine-rich repeat (NBS-LRR) genes in Brassicaceae
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揭示十字花科核苷酸结合位点富含亮氨酸重复序列（NBS-LRR）基因的动态进化
DOI：
10.1111/jipb.12365
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发表时间：
2016
影响因子：
11.4
通讯作者：
Chen Jian-Qun
中科院分区：
文献类型：
--
作者：
Zhang Yan-Mei;Shao Zhu-Qing;Wang Qiang;Hang Yue-Yu;Xue Jia-Yu;Wang Bin;Chen Jian-Qun
植物基因组包含数十至数百个核苷酸结合位点-亮氨酸丰富重复序列（NBS - LRR）基因；然而，这些抗性基因的长期进化历史尚未完全了解。本研究以5个芸苔科基因组和番木瓜基因组为研究对象，探讨在过去7200万年中，这个蔷薇II谱系中NBS‐LRR基因的变化。从拟南芥（marabidopsis lyrata, 1998），A。蓝藻（165），油菜（204），风疹芥（127），salsuginethellungiella(88)，和c。木瓜(51)。在每个基因组中，NBS‐LRR基因在染色体间的分布不均匀，且大部分聚集在一起。系统发育分析表明，在十字花科物种形成事件发生前后，toll/interleukin - 1受体- NBS - LRR （TNL）基因和非toll/interleukin - 1受体- NBS - LRR （nTNL）基因均表现出先扩张后收缩的模式，表明NBS - LRR基因和NBS - LRR基因亚类是同步响应病原体压力的。此外，通过检查TNL和nTNL基因在不同进化节点上的获得/丢失，本研究揭示了这两种事件在TNL基因中往往发生得更剧烈。最后，nTNL基因的系统发育表明，NBS - LRR亚类由两个独立的古老基因类型组成：RPW8 - NBS - LRR和Coiled - coil - NBS - LRR。
Plant genomes harbor dozens to hundreds of nucleotide‐binding site‐leucine‐rich repeat (NBS‐LRR) genes; however, the long‐term evolutionary history of these resistance genes has not been fully understood. This study focuses on five Brassicaceae genomes and theCarica papayagenome to explore changes in NBS‐LRR genes that have taken place in this Rosid II lineage during the past 72 million years. Various numbers of NBS‐LRR genes were identified fromArabidopsis lyrata(198),A. thaliana(165),Brassica rapa(204),Capsella rubella(127),Thellungiella salsuginea(88), andC. papaya(51). In each genome, the identified NBS‐LRR genes were found to be unevenly distributed among chromosomes and most of them were clustered together. Phylogenetic analysis revealed that, before and after Brassicaceae speciation events, both toll/interleukin‐1 receptor‐NBS‐LRR (TNL) genes and non‐toll/interleukin‐1 receptor‐NBS‐LRR (nTNL) genes exhibited a pattern of first expansion and then contraction, suggesting that both subclasses of NBS‐LRR genes were responding to pathogen pressures synchronically. Further, by examining the gain/loss of TNL and nTNL genes at different evolutionary nodes, this study revealed that both events often occurred more drastically in TNL genes. Finally, the phylogeny of nTNL genes suggested that this NBS‐LRR subclass is composed of two separate ancient gene types: RPW8‐NBS‐LRR and Coiled‐coil‐NBS‐LRR.