GLUCONATE METABOLISM IN ESCHERICHIA COLI
GLUCONATE METABOLISM IN ESCHERICHIA COLI
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DOI：
10.1128/jb.93.3.941-949.1967
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发表时间：
1967-01-01
影响因子：
3.2
通讯作者：
DOBROGOSZ, WJ
中科院分区：
文献类型：
--
作者：
EISENBERG, RC;DOBROGOSZ, WJ
根据文献中提供的信息，大肠杆菌中的葡萄糖酸异化被认为是通过己糖单磷酸途径发生的。本研究提供的证据表明，葡萄糖酸在该生物体中通过诱导型 Entner-Doudoroff 途径进行分解代谢。该证据基于对 Cl4 标记的葡萄糖酸盐或葡萄糖产生的最终产品的色谱检查、Cl4 在由专门标记的 C14-葡萄糖和 C14-葡萄糖酸盐形成的丙酮酸盐碳原子中的分布，以及无细胞提取物从 6-磷酸葡萄糖酸盐产生丙酮酸盐的能力。 Entner-Doudoroff 途径对葡萄糖酸盐的降解与葡萄糖的糖酵解同时发生。讨论了大肠杆菌和其他细菌中葡萄糖酸诱导的 Entner-Doudoroff 途径活性与葡萄糖分解代谢之间的关系。
On the basis of information available in the literature, gluconate dissimilation in E. coli is thought to occur via the hexose monophosphate pathway. Evidence is presented in this study that gluconate is catabolized in this organism via an inducible Entner-Doudoroff pathway. This evidence is based on chromatographic examination of end products produced from Cl4-labeled gluconate or glucose, distribution of Cl4 in the carbon atoms of pyruvate formed from specifically labeled C14-glucose and C14-gluconate, and the ability of cell-free extracts to produce pyruvate from 6-phosphogluco-nate. Degradation of gluconate by an Entner-Doudoroff pathway occurred simultaneously with a glycolytic cleavage of glucose. A relationship between gluconate-induced, Entner-Doudoroff pathway activity and catabolism of glucose in E. coli and other bacterial species is discussed.