NIGMS human/rodent somatic cell hybrid mapping panels 1 and 2.
NIGMS human/rodent somatic cell hybrid mapping panels 1 and 2.
复制标题
NIGMS 人类/啮齿动物体细胞杂交图谱 1 和 2。
DOI：
10.1006/geno.1993.1190
复制
发表时间：
1993
期刊：
影响因子：
4.4
通讯作者：
Mulivor,RA
中科院分区：
文献类型：
--
作者：
Drwinga,HL;Toji,LH;Kim,CH;Greene,AE;Mulivor,RA
NIGMS 人类遗传突变细胞存储库目前正在分发两个特征良好的人类/啮齿动物体细胞混合绘图面板。作图第 1 组由从保留 1 至 19 条人类染色体的 18 个杂交细胞培养物中分离出的 DNA 组成。作图第 2 组包含来自保留 1 或 2 条人类染色体的杂交体的 DNA。除两个杂交体外，所有杂交体都保留了一条完整的人类染色体。 Mapping Panel 2 也可作为细胞培养物提供。这些资源对于人类基因组计划计划来说应该是有价值的。本文描述了杂交细胞培养物的来源以及用于制备和表征面板的程序。
The NIGMS Human Genetic Mutant Cell Repository is currently distributing two well-characterized human/rodent somatic cell hybrid mapping panels. Mapping Panel 1 consists of DNA isolated from 18 hybrid cell cultures retaining from 1 to 19 human chromosomes. Mapping Panel 2 contains DNA from hybrids retaining 1 or 2 human chromosomes. All but 2 of the hybrids retain a single intact human chromosome. Mapping Panel 2 is also available as cell cultures. These resources should prove valuable to the Human Genome Project initiative. This article describes the sources of the hybrid cell cultures and the procedures utilized to prepare and characterize the panels.