Interleukin-19 upregulates keratinocyte growth factor and is associated with psoriasis
Interleukin-19 upregulates keratinocyte growth factor and is associated with psoriasis
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DOI：
10.1111/j.1365-2133.2005.06665.x
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发表时间：
2005-09-01
影响因子：
10.3
通讯作者：
Chang, MS
中科院分区：
文献类型：
--
作者：
Li, HH;Lin, YC;Chang, MS
背景IL-19是IL-10家族的一员，通过IL-20R1/IL-20R2异源二聚体传递信号，参与角质形成细胞的异常分化和增殖。目的探讨白介素19(IL-19)在银屑病发病过程中的作用。方法采用聚合酶链式反应技术检测IL-19作用下CD8+T细胞角质形成细胞生长因子(KGF)转录产物的表达。接下来，我们开发了单抗和多克隆抗体来检测银屑病患者和健康志愿者血清中IL-19的水平。结果IL-19可上调CD8+T细胞表面KGF的转录水平。银屑病患者血清IL-19水平低于健康志愿者。两组间差异有统计学意义(P<；0.05)。结论IL-19在银屑病复杂的细胞因子网络中起重要作用。
Background Interleukin (IL)-19, a member of the IL-10 family, signals through the IL-20R1/IL-20R2 heterodimer, which is shown to be involved in abnormal keratinocyte differentiation and proliferation. Little is known about its in vitro biological functions or its role in psoriasis.Objectives To investigate the role of IL-19 in the psoriatic process.Methods The expression of keratinocyte growth factor (KGF) transcripts was measured by polymerase chain reaction in CD8+ T cells treated with IL-19. Next, we developed monoclonal and polyclonal antibodies to measure the levels of IL-19 in the sera of patients with psoriasis and healthy volunteers using an enzyme-linked immunosorbent assay. In addition, we performed immunohistochemical staining on psoriatic skin and normal controls.Results We found that IL-19 upregulated KGF transcripts on CD8+ T cells. Patients with psoriasis had a lower level of IL-19 in serum than healthy volunteers. The difference between these two groups was statistically significant (P < 0.05). IL-19 expression was seen in basal and suprabasal keratinocytes in a continuous pattern, and was increased in psoriatic epidermis.Conclusions These results suggest that IL-19 plays a role in the complex pathological cytokine network in psoriasis.