Diversity and evolution of avian influenza viruses in live poultry markets, free-range poultry and wild wetland birds in China
Diversity and evolution of avian influenza viruses in live poultry markets, free-range poultry and wild wetland birds in China
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DOI：
10.1099/jgv.0.000399
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发表时间：
2016-04-01
影响因子：
3.8
通讯作者：
Zhang, Yong-Zhen
中科院分区：
文献类型：
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作者：
Chen, Liang-Jun;Lin, Xian-Dan;Zhang, Yong-Zhen
新型禽流感病毒（AIV）的广泛传播凸显了中国出现大流行性流感的风险。为了解不同生态环境下禽流感病毒的流行情况和遗传多样性，我们对浙江省和湖北省的活禽市场、散养家禽和湿地野生鸟类生境中的禽流感病毒进行了调查。值得注意的是，LPMs包含AIV感染的最高频率，以及最多的亚型（n=9）和亚型共感染（n=14），以及频繁的重配，这表明它们在助长AIV传播中起着积极的作用。在两省的野生鸟类和湖北省靠近湿地地区的一个采样点的散养家禽中也发现了AIV阳性样本。H9N2、H7N9和H5N1是浙江省LPM中最常见的亚型，H5N6和H9N2是湖北省LPM中的优势亚型。对43株禽流感病毒全基因组序列的系统发育分析显示，在这两个地理区域的LMP中流行着三种相关的H5亚型。值得注意的是，从湿地地区和自由放养的家禽中取样的病毒含有复杂的抑制剂，其中一些片段的来源尚不清楚。总体而言，我们的研究强调了中国中部和东南部两个人口稠密地区AIV遗传多样性的程度，特别是在LPMs，并强调需要持续监测。
The wide circulation of novel avian influenza viruses (AIVs) highlights the risk of pandemic influenza emergence in China. To investigate the prevalence and genetic diversity of AIVs in different ecological contexts, we surveyed AIVs in live poultry markets (LPMs), free-range poultry and the wetland habitats of wild birds in Zhejiang and Hubei provinces. Notably, LPMs contained the highest frequency of AIV infection, and the greatest number of subtypes (n=9) and subtype co-infections (n=14), as well as frequent reassortment, suggesting that they play an active role in fuelling AIV transmission. AIV-positive samples were also identified in wild birds in both provinces and free-range poultry in one sampling site close to a wetland region in Hubei. H9N2, H7N9 and H5N1 were the most commonly sampled subtypes in the LPMs from Zhejiang, whilst H5N6 and H9N2 were the dominant subtypes in the LPMs from Hubei. Phylogenetic analyses of the whole-genome sequences of 43 AIVs revealed that three reassortant H5 subtypes were circulating in LMPs in both geographical regions. Notably, the viruses sampled from the wetland regions and free-range poultry contained complex reassortants, for which the origins of some segments were unclear. Overall, our study highlights the extent of AIV genetic diversity in two highly populated parts of central and south-eastern China, particularly in LPMs, and emphasizes the need for continual surveillance.