Poplar GATA transcription factor PdGNC is capable of regulating chloroplast ultrastructure, photosynthesis, and vegetative growth in Arabidopsis under varying nitrogen levels
Poplar GATA transcription factor PdGNC is capable of regulating chloroplast ultrastructure, photosynthesis, and vegetative growth in Arabidopsis under varying nitrogen levels
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杨树GATA转录因子PdGNC能够在不同氮水平下调节拟南芥叶绿体超微结构、光合作用和营养生长
DOI：
10.1007/s11240-014-0536-y
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发表时间：
2014-11-01
影响因子：
3
通讯作者：
Yin, Weilun
中科院分区：
文献类型：
--
作者：
An, Yi;Han, Xiao;Yin, Weilun
光合作用和氮素有效性对生物量的积累至关重要。GATA转录因子家族在叶绿体发育和氮代谢中发挥重要作用。在这里，我们首次从速生杨树无性系NE-19中克隆了GATA转录因子PdGNC。在高硝酸盐、充足硝酸盐和低硝酸盐(LN)条件下，PdGNC的高表达结果支持PdGNC增加了叶片和茎中每细胞的叶绿体数量和大小，提高了26.12%的叶绿素水平，并表现出最高的淀粉含量。PdGNC的过表达还通过增加基粒和类囊体数对叶绿体超微结构产生明显影响。通过改变叶绿素荧光参数Fv/F0、Fv/Fm、QP、NPQ和ΦPSⅡ，转基因LN株系的光合作用速率比野生型提高了42.17%。在形态上，PdGNC促进了LN较长的主根和较大的叶面积，并表现出较高的相对生长速率。总之，PdGNC在低硝酸盐水平下提高了植物的光合作用能力和生长，因此，它可能被用于转基因育种，以提高有限氮素条件下的硝酸盐利用和植物生长速率。
Photosynthesis and nitrogen availability are crucial to the accumulation of biomass. The GATA transcription factor family plays important roles in chloroplast development and nitrogen metabolism. Here, we cloned, for the first time, the GATA transcription factor PdGNC from the fast-growing poplar clone NE-19. The overexpression results from Arabidopsis under high nitrate, sufficient nitrate, and low nitrate (LN) support that PdGNC increased the chloroplast number and size per cell in leaf and stem, improved the chlorophyll level by 26.12 % and exhibited the highest starch content in LN. Overexpression of PdGNC also had pronounced effects on chloroplast ultrastructure by increasing the number of grana and thylakoids. The photosynthetic rate in transgenic LN lines was 42.17 % higher than in the wild type through modification of the chlorophyll fluorescence parameters Fv/F0, Fv/Fm, qP, NPQ, and ΦPSII. Morphologically, PdGNC promoted longer primary roots and larger leaf areas, and exhibited a higher relative growth rate in LN. Altogether, PdGNC improved photosynthetic capacity and plant growth under low nitrate levels; thus, it could potentially be used in transgenic breeding to improve nitrate utilization and plant growth rates under limited nitrogen conditions.