Phylogenetic analyses of mtDNA sequences reveal three cryptic lineages in the widespread neotropical frog Leptodactylus fuscus (Schneider, 1799) (Anura, Leptodactylidae)
Phylogenetic analyses of mtDNA sequences reveal three cryptic lineages in the widespread neotropical frog Leptodactylus fuscus (Schneider, 1799) (Anura, Leptodactylidae)
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DOI：
10.1111/j.1095-8312.2006.00581.x
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发表时间：
2006-02-01
影响因子：
1.9
通讯作者：
Heyer, WR
中科院分区：
文献类型：
--
作者：
Camargo, A;De Sá, RO;Heyer, WR
细趾蛙（Leptodactylus fuscus）是一种分布于巴拿马至阿根廷、安第斯山脉以东地区的新热带蛙，也栖息于特立尼达岛、玛格丽塔岛和托巴哥群岛。我们对在L.地理分布范围内收集的标本的12 S rRNA、16 S rRNA、tRNA-Leu和ND 1线粒体（mt）DNA序列进行了系统发育分析。fuscus检验两个备选假设：（i）L. fuscus是一个单一的，广泛分布的物种，或（ii）L。fuscus是一个物种复合体。我们对mtDNA分支间的地理关联和遗传变异的划分进行了统计学检验。线粒体DNA数据支持L. fuscus未连锁的mtDNA和核标记独立支持一个“北方”系统发育单位的独特性。此外，线粒体DNA数据划分为两个分支，没有姐妹关系，相互之间，与高分化和缺乏基因流的南方种群之间的等位酶数据的建议。线粒体DNA和等位酶谱的一致性表明，在L.没有形态或叫声分化的褐色。这项研究说明了一个情况下，在物种形成过程中的谱系分裂发生在没有分歧的生殖隔离机制（如广告呼吁青蛙），相反的预期使用生物物种框架预测。(c)2006 The Linnean Society of伦敦，Biological Journal of the Linnean Society，2006，87，325-341.没有要求美国政府的原始作品。
Leptodactylus fuscus is a neotropical frog ranging from Panama to Argentina, to the east of the Andes mountains, and also inhabiting Margarita, Trinidad, and the Tobago islands. We performed phylogenetic analyses of 12S rRNA, 16S rRNA, tRNA-Leu, and ND1 mitochondrial (mt) DNA sequences from specimens collected across the geographic distribution of L. fuscus to examine two alternative hypotheses: (i) L. fuscus is a single, widely distributed species, or (ii) L. fuscus is a species complex. We tested statistically for geographic association and partitioning of genetic variation among mtDNA clades. The mtDNA data supported the hypothesis of several cryptic species within L. fuscus. Unlinked mtDNA and nuclear markers supported independently the distinctness of a 'northern' phylogenetic unit. In addition, the mtDNA data divided the southern populations into two clades that showed no sister relationship to each other, consistent with high differentiation and lack of gene flow among southern populations as suggested by allozyme data. Concordance between mtDNA and allozyme patterns suggests that cryptic speciation has occurred in L. fuscus without morphological or call differentiation. This study illustrates a case in which lineage splitting during the speciation process took place without divergence in reproductive isolation mechanisms (e.g. advertisement call in frogs), contrary to expectations predicted using a biological species framework. (c) 2006 The Linnean Society of London, Biological Journal of the Linnean Society, 2006, 87, 325-341. No claim to original US government works.