Covalent display of oligosaccharide arrays in microtiter plates
Covalent display of oligosaccharide arrays in microtiter plates
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DOI：
10.1021/ja048433f
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发表时间：
2004-07-21
影响因子：
15
通讯作者：
Wong, CH
中科院分区：
文献类型：
--
作者：
Bryan, MC;Fazio, F;Wong, CH
一种用于在微量滴定板中显示复杂寡糖的共价阵列已经被开发出来。这种策略有助于将碳水化合物展示给感兴趣的蛋白质，例如凝集素和抗体，包括针对HIV包膜寡甘露糖的广泛中和抗体2G12，并且可以从表面切割以通过质谱法进行进一步表征。该系统用于探测2G12与最佳表位（Kd 0.1 μM）的多价相互作用。
A covalent array for the display of complex oligosaccharides in microtiter plates has been developed. This strategy is conducive to the display of carbohydrates to proteins of interest such as lectins and antibodies, including the broadly neutralizing antibody 2G12 against HIV envelope oligomannose and can be cleaved from the surface for further characterization by mass spectrometry. The system was used to probe the multivalent interaction of 2G12 with an optimal epitope (Kd0.1 μM).