Salmonella Typhimurium biofilm disruption by a human antibody that binds a pan-amyloid epitope on curli
Salmonella Typhimurium biofilm disruption by a human antibody that binds a pan-amyloid epitope on curli
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DOI：
10.1038/s41467-020-14685-3
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发表时间：
2020-02-21
影响因子：
16.6
通讯作者：
Tukel, Cagla
中科院分区：
文献类型：
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作者：
Tursi, Sarah A.;Puligedda, Rama Devudu;Tukel, Cagla
细菌生物膜，特别是与植入的医疗器械相关的细菌生物膜，难以根除。卷曲淀粉样纤维是肠杆菌科细菌生物膜的重要组成部分。在这里，我们表明，具有泛淀粉样蛋白结合活性的人单克隆抗体（单克隆抗体3H3）可以破坏肠道沙门氏菌血清型鼠伤寒沙门氏菌在体外和体内形成的生物膜。抗体破坏生物膜结构，增强抗生素和免疫细胞对生物膜的根除。在小鼠中，3H3注射允许导管相关S.鼠伤寒生物膜。因此，结合泛淀粉样蛋白表位的单克隆抗体具有预防或根除细菌生物膜的潜力。
Bacterial biofilms, especially those associated with implanted medical devices, are difficult to eradicate. Curli amyloid fibers are important components of the biofilms formed by the Enterobacteriaceae family. Here, we show that a human monoclonal antibody with pan-amyloid-binding activity (mAb 3H3) can disrupt biofilms formed by Salmonella enterica serovar Typhimurium in vitro and in vivo. The antibody disrupts the biofilm structure, enhancing biofilm eradication by antibiotics and immune cells. In mice, 3H3 injections allow antibiotic-mediated clearance of catheter-associated S. Typhimurium biofilms. Thus, monoclonal antibodies that bind a pan-amyloid epitope have potential to prevent or eradicate bacterial biofilms.