Silicon Nitride Inactivates SARS-CoV-2 in vitro
Silicon Nitride Inactivates SARS-CoV-2 in vitro
复制标题
氮化硅在体外灭活 SARS-CoV-2
DOI：
10.1101/2020.08.29.271015
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
R. Bock
中科院分区：
文献类型：
--
作者：
C. Lehman;Rafaela Flur;K. Kehn;B. McEntire;B. Bal;R. Bock
引起COVID-19大流行的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）仍然存在，因此对几种材料具有潜在的传染性。阻止污染物介导的COVID-19传播的一种策略是开发表面化学性质可以自发抑制SARS-CoV-2的材料。氮化硅（Si 3 N4），一种用于脊柱融合手术的材料，就是这样一种候选材料，因为它已被证明可以抑制几种细菌和病毒株。这项研究假设与Si 3 N4接触会使SARS-CoV-2死亡，而哺乳动物细胞不会受到影响。材料SARS-CoV-2病毒粒子（2×104 PFU/mL，在生长培养基中稀释）分别暴露于5、10、15和20%（w/v）烧结Si 3 N4颗粒的水悬浮液中1、5和10分钟。在暴露于病毒之前，在暴露后24和48小时在Vero细胞中评估单独Si 3 N4的细胞毒性测试。每次暴露于Si 3 N4后，通过空斑测定定量剩余的感染性病毒。结果在5%和10%（w/v）浓度的Si 3 N4下，暴露时间长达10分钟时，Vero细胞活力增加，并且在高达20%（w/v）时，对细胞健康和活力的影响极小。然而，当暴露于所有浓度的Si 3 N4时，SARS-CoV-2滴度显著降低;病毒滴度的降低在85% -99.6%之间，取决于暴露的剂量和持续时间。结论Si 3 N4对Vero细胞无毒性，但对SARS-CoV-2有较强的抗病毒活性。杀病毒效果随着Si 3 N4浓度的增加和暴露时间的延长而增加。基于Si 3 N4的表面处理策略可以提供新的方法来阻止SARS-CoV-2在表面上的持久性和传染性，并阻止COVID-19的传播。
Introduction Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is responsible for the COVID-19 pandemic, remains viable and therefore potentially infectious on several materials. One strategy to discourage the fomite-mediated spread of COVID-19 is the development of materials whose surface chemistry can spontaneously inactivate SARS-CoV-2. Silicon nitride (Si3N4), a material used in spine fusion surgery, is one such candidate because it has been shown to inactivate several bacterial species and viral strains. This study hypothesized that contact with Si3N4 would inactivate SARS-CoV-2, while mammalian cells would remain unaffected. Materials SARS-CoV-2 virions (2×104 PFU/mL diluted in growth media) were exposed to 5, 10, 15, and 20% (w/v) of an aqueous suspension of sintered Si3N4 particles for durations of 1, 5, and 10 minutes, respectively. Before exposure to the virus, cytotoxicity testing of Si3N4 alone was assessed in Vero cells at 24 and 48 hour post-exposure times. Following each exposure to Si3N4, the remaining infectious virus was quantitated by plaque assay. Results Vero cell viability increased at 5% and 10% (w/v) concentrations of Si3N4 at exposure times up to 10 minutes, and there was only minimal impact on cell health and viability up to 20% (w/v). However, the SARS-CoV-2 titers were markedly reduced when exposed to all concentrations of Si3N4; the reduction in viral titers was between 85% - 99.6%, depending on the dose and duration of exposure. Conclusions Si3N4 was non-toxic to the Vero cells while showing strong antiviral activity against SARS-CoV-2. The viricidal effect increased with increasing concentrations of Si3N4 and longer duration of exposure. Surface treatment strategies based on Si3N4 may offer novel methods to discourage SARS-CoV-2 persistence and infectivity on surfaces and discourage the spread of COVID-19.
影响因子：
7.3
作者：
Marin, E.;Boschetto, F.;Pezzotti, G.
通讯作者：
Pezzotti, G.