Further electron microscope studies of a mouse leukemia induced by cell-free filtrates.
Further electron microscope studies of a mouse leukemia induced by cell-free filtrates.
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对无细胞滤液诱导的小鼠白血病的进一步电子显微镜研究。
DOI：
10.1083/jcb.7.4.747
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发表时间：
1960-07
期刊：
影响因子：
--
通讯作者：
FRIEND, C
中科院分区：
文献类型：
--
作者：
DE HARVEN, E;FRIEND, C
进一步的电镜观察，从瑞士和DBA/2小鼠白血病传播无细胞滤液到成年动物。研究了白血病细胞的细胞学特征、病毒的精细结构以及病毒与宿主细胞的关系。白血病病毒的外径平均为870 A，似乎是通过出芽过程在细胞膜水平形成的。病毒最常在细胞间隙中观察到，但也常在巨核细胞的细胞质液泡中发现。采用氢氧化铅染色法对白血病物质进行研究。已发现病毒对这种铅盐具有相当的亲和力，其强度仅与RNP颗粒对同一化学物质的亲和力相当。
Further electron microscope observations of tissues from Swiss and DBA/2 mice with leukemia transmissible by cell-free filtrates to the adult animals are presented. The cytological characteristics of the leukemic cells, the fine structure of the viruses, and the virus host-cell relationships have been examined. The leukemia virus has an external diameter averaging 870 A, and appears to be formed at the level of cell membranes by a budding process. The viruses are observed most frequently in intercellular spaces, but are also often found within cytoplasmic vacuoles of megakaryocytes. Lead hydroxide staining was applied to the study of the leukemic material. The viruses have been found to have a considerable affinity for this lead salt, only comparable in intensity to the affinity shown by RNP granules for the same chemical.