Behavior of cellulose production of Acetobacter xylinum in 13C-enriched cultivation media including movements on nematic ordered cellulose templates
Behavior of cellulose production of Acetobacter xylinum in 13C-enriched cultivation media including movements on nematic ordered cellulose templates
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DOI：
10.1016/j.carbpol.2005.02.018
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发表时间：
2005-06-20
影响因子：
11.2
通讯作者：
Kondo, T
中科院分区：
文献类型：
--
作者：
Hesse, S;Kondo, T
研究了木醋杆菌NQ-5(ATCC 53582)和AY-201(ATCC 23769)在含有天然C-13丰度为1.1%的D-葡萄糖的Hestrin-Schramm培养基中的细胞分裂率和细菌运动情况。在D-葡萄糖-U-C-13(6)存在下进行了比较研究，C-13均一标记99%作为唯一碳源。在富含C-13的培养基中，NQ-5和AY-201的细菌生长速率分别提高了约13%和26%。利用光学显微镜实时视频分析技术，研究了纤维素纳米纤维在向列相有序纤维素(NOC)模板上的分泌和沉积反作用力引起的单细胞运动。结果，D-葡萄糖-U-C-13(6)降低了两个菌株的运动速度，这与细胞分裂中上述生长速度的趋势相反。CP/MAS C-13核磁共振和近红外傅立叶变换拉曼光谱测试表明，沉积的纤维素纤维为纤维素晶态，尽管生物合成和沉积后的纤维素与NOC表面有很强的相互作用。(C)2005爱思唯尔有限公司。保留所有权利。
Acetobacter xylinum NQ-5 (ATCC 53582) and AY-201 (ATCC 23769) cultivated in the Hestrin-Schramm medium containing D-glucose with a natural C-13-abundance of 1.1% were investigated regarding their cell division rates and the bacterial movements. Comparative studies were carried out in the presence Of D-glucose-U-C-13(6) with a uniform C-13-labeling of 99% as the sole carbon source. The bacterial growth rates in numbers were found to increase in the C-13-enriched media by about 13% for NQ-5 and 26% for AY-201, respectively. The movements of single cells caused by the inverse force of the secretion and deposition of cellulose nanofibers on nematic ordered cellulose (NOC) templates were investigated by real-time video analyzes using light microscopy. As a result, D-glucose-U-C-13(6) reduced the speed of motion for both strains, which was an opposite trend to the above growth rates in the cell division. The deposited cellulose fibers were proved to be in a cellulose crystalline form by CP/MAS C-13 NMR and NIR FT Raman spectroscopic measurements, although the biosynthesized and thereafter-deposited cellulose has a strong interaction with the surface of NOC. (c) 2005 Elsevier Ltd. All rights reserved.