Novel bacteria degrading N-acylhomoserine lactones and their use as quenchers of quorum-sensing-regulated functions of plant-pathogenic bacteria
Novel bacteria degrading N-acylhomoserine lactones and their use as quenchers of quorum-sensing-regulated functions of plant-pathogenic bacteria
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DOI：
10.1099/mic.0.26375-0
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发表时间：
2003-08-01
期刊：
影响因子：
2.8
通讯作者：
Dessaux, Y
中科院分区：
文献类型：
--
作者：
Uroz, S;D'Angelo-Picard, C;Dessaux, Y
从烟草根际土壤中分离到能降解群体感应信号分子N-己酰高丝氨酸内酯的细菌。根据基因组REP-PCR和rrs PCR-RFLP图谱，25株降解高丝氨酸内酯的菌株分为6组。来自每组的代表性菌株被鉴定为假单胞菌属、丛毛单胞菌属、Variovorax属和红球菌属的成员。所有这些菌株降解N-酰基高丝氨酸内酯以外的己酸衍生物，虽然具有不同的特异性和动力学。这些分离株之一，红串红球菌菌株W2，用于淬灭OS调节的其他微生物的功能。在体外，W2强烈干扰紫色色杆菌产生紫色素，并干扰根癌农杆菌的致病性转移。因普兰塔河红串菌W2显著降低了胡萝卜果胶杆菌胡萝卜亚种的致病性。马铃薯块茎中的胡萝卜素。这些结果揭示了根际QS干扰细菌的多样性，并证明了利用天然细菌分离物靶向OS信号分子来控制病原体的有效性。
Bacteria degrading the quorum-sensing (OS) signal molecule N-hexanoylhomoserine lactone were isolated from a tobacco rhizosphere. Twenty-five isolates degrading this homoserine lactone fell into six groups according to their genomic REP-PCR and rrs PCR-RFLP profiles. Representative strains from each group were identified as members of the genera Pseudomonas, Comamonas, Variovorax and Rhodococcus. All these isolates degraded N-acylhomoserine lactones other than the hexanoic acid derivative, albeit with different specificity and kinetics. One of these isolates, Rhodococcus erythropolis strain W2, was used to quench OS-regulated functions of other microbes. In vitro, W2 strongly interfered with violacein production by Chromobacterium violaceum, and transfer of pathogenicity in Agrobacterium tumefaciens. In planta, R. erythropolis W2 markedly reduced the pathogenicity of Pectobacterium carotovorum subsp. carotovorum in potato tubers. These series of results reveal the diversity of the QS-interfering bacteria in the rhizosphere and demonstrate the validity of targeting OS signal molecules to control pathogens with natural bacterial isolates.