The Microbiome of Potentially Malignant Oral Leukoplakia Exhibits Enrichment for Fusobacterium, Leptotrichia, Campylobacter, and Rothia Species.
The Microbiome of Potentially Malignant Oral Leukoplakia Exhibits Enrichment for Fusobacterium, Leptotrichia, Campylobacter, and Rothia Species.
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DOI：
10.3389/fmicb.2017.02391
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发表时间：
2017
影响因子：
5.2
通讯作者：
Moran GP
中科院分区：
文献类型：
--
作者：
Amer A;Galvin S;Healy CM;Moran GP
口腔白斑表现为口腔粘膜上的白色斑块，被认为具有显著的恶性潜在性。虽然这些斑块中白色念珠菌的定植很常见，但人们对这些斑块的细菌微生物区系知之甚少。在目前的研究中，我们分析了36名口腔白斑患者的微生物组，并与来自相同患者和健康对照组的健康黏膜组织进行了比较，以确定是否可以在恶变过程的早期发现特定的微生物富集物，这可能在疾病的发展中发挥作用。这是通过使用Illumina MiSeq对细菌16S rRNA基因的V1-V2区进行序列分析进行的。口腔白斑表现为梭菌数量增加，细菌数量减少(Metastats，P<；0.01)。白斑患者中念珠菌定植的发生率也高于健康对照组(P=0.025)。口腔白斑的细菌定植模式多种多样，可辨别出5个不同的细菌聚集区。这些集群显示了梭杆菌、细毛杆菌和弯曲杆菌物种(Pearson P<；0.01)的共存，这与在结直肠癌上观察到的微生物共存模式惊人地相似。舌部白斑患者的口腔白斑组织中产乙醛微生物明显增多(P<0.0012)。严重的异型增生与细毛菌水平升高有关。和简约弯曲杆菌(P<；0.05)。口腔白斑表现出一种改变的微生物群，与结直肠癌的微生物群有相似之处。
Oral leukoplakia presents as a white patch on the oral mucosa and is recognized as having significant malignant potential. Although colonization of these patches with Candida albicans is common, little is known about the bacterial microbiota of these patches. In the current study we analyzed the microbiome of oral leukoplakia in 36 patients compared to healthy mucosal tissue from the same patients and healthy control subjects to determine if specific microbial enrichments could be identified early in the malignant process that could play a role in the progression of the disease. This was carried out by sequence analysis of the V1–V2 region of the bacterial 16S rRNA gene using the Illumina MiSeq. Oral leukoplakia exhibited increased abundance of Fusobacteria and reduced levels of Firmicutes (Metastats P < 0.01). Candida colonization was also more prevalent in leukoplakia patients relative to healthy controls (P = 0.025). Bacterial colonization patterns on oral leukoplakia were highly variable and five distinct bacterial clusters were discerned. These clusters exhibited co-occurrence of Fusobacterium, Leptotrichia, and Campylobacter species (Pearson P < 0.01), which is strikingly similar to the microbial co-occurrence patterns observed on colorectal cancers. Increased abundance of the acetaldehydogenic microorganism Rothia mucilaginosa was also apparent on oral leukoplakias from lingual sites (P 0.0012). Severe dysplasia was associated with elevated levels of Leptotrichia spp. and Campylobacter concisus (P < 0.05). Oral leukoplakia exhibits an altered microbiota that has similarities to the microbiome of colorectal cancer.