Endometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry.
Endometriosis foci differentiation by rapid lipid profiling using tissue spray ionization and high resolution mass spectrometry.
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DOI：
10.1038/s41598-017-02708-x
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发表时间：
2017-05-31
影响因子：
4.6
通讯作者：
Sukhikh GT
中科院分区：
文献类型：
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作者：
Chagovets VV;Wang Z;Kononikhin AS;Starodubtseva NL;Borisova A;Salimova D;Popov IA;Kozachenko AV;Chingin K;Chen H;Frankevich VE;Adamyan LV;Sukhikh GT
获得关于组织样本分子组成的快速筛选信息对于疾病生物标记物搜索和在线手术控制非常重要。本研究采用直接组织喷雾质谱仪对90例在位和异位内膜组织进行了正负离子模式的高分辨质谱分析。这两种离子模式中最丰富的峰是对应于脂类的那些峰。观察到三种脂类：磷脂酰胆碱(PC)、鞘磷脂(SM)和磷脂乙醇胺(PE)。直接组织分析主要提供了正离子模式下的PC和SM脂(29种)和负离子模式下的PC、SM和PE脂(50种)的信息，为子宫内膜异位症病灶的鉴别提供了补充数据。磷脂与多不饱和酰基和烷基的差异最大。尽管组织喷雾被认为是组织研究的合适工具，但由于其复杂性较高，可能形成的加合物较多，必须考虑多种干扰，因此应注意对质谱学的解释。本工作扩展了直接组织分析的应用范围，以快速区分不同部位的子宫内膜异位症组织。
Obtaining fast screening information on molecular composition of a tissue sample is of great importance for a disease biomarkers search and for online surgery control. In this study, high resolution mass spectrometry analysis of eutopic and ectopic endometrium tissues (90 samples) is done using direct tissue spray mass spectrometry in both positive and negative ion modes. The most abundant peaks in the both ion modes are those corresponding to lipids. Species of three lipid classes are observed, phosphatidylcholines (PC), sphingomyelins (SM) and phosphoethanolamines (PE). Direct tissue analysis gives mainly information on PC and SM lipids (29 species) in positive ion mode and PC, SM and PE lipids (50 species) in negative ion mode which gives complementary data for endometriosis foci differentiation. The biggest differences were found for phospholipids with polyunsaturated acyls and alkils. Although, tissue spray shows itself as appropriate tool for tissue investigation, caution should be paid to the interpretation of mass spectra because of their higher complexity with more possible adducts formation and multiple interferences must be taken into account. The present work extends the application of direct tissue analysis for the rapid differentiation between endometriotic tissues of different foci.