Correlation between expression of platelet-derived endothelial cell growth factor (Thymidine phosphorylase) and microvessel density in early-stage human colon carcinomas
Correlation between expression of platelet-derived endothelial cell growth factor (Thymidine phosphorylase) and microvessel density in early-stage human colon carcinomas
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DOI：
10.1093/jjco/27.4.227
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发表时间：
1997-08-01
影响因子：
2.4
通讯作者：
Moriwaki, S
中科院分区：
文献类型：
--
作者：
Saeki, T;Tanada, M;Moriwaki, S
血小板源性内皮细胞生长因子可能通过其血管生成作用在肿瘤发展中发挥作用。为了阐明血小板源性内皮细胞生长因子的表达和微血管密度在人类结肠癌的发展之间的关系，我们研究了80例早期结肠癌，用显微镜和免疫组化。血小板源性内皮细胞生长因子的定位通过免疫细胞化学进行评估，而微血管计数通过HE染色或因子VIII免疫染色进行评价。在所检查的癌中，35例被归类为m癌，包括原位癌，而45例被归类为sm癌，15（42.9%）的35个月和30个月45例SM癌中66.7%血管密度增高，20例15例（33.3%）sm癌血管密度高于m癌，浸润深度与血管密度有显著相关性。在45例高度血管化的肺癌中，44例表达血小板源性内皮细胞生长因子，其表达频率与微血管密度有显著相关性（P = 0.012）。这些数据表明，微血管密度可能与癌症浸润的深度和血小板衍生的内皮细胞生长因子可能通过血管生成在结肠癌发展的早期阶段发挥重要作用。
Platelet-derived endothelial cell growth factor may play a role in tumor development through its angiogenic action. To clarify the relationship between expression of platelet-derived endothelial cell growth factor and microvessel density in the development of human colon carcinoma, we examined 80 early-stage colon carcinomas using microscopy and immunohistochemistry. Localization of platelet-derived endothelial cell growth factor was assessed by immunocytochemistry, while microvessel count was evaluated by either HE staining or Factor VIII immunostaining. Among the examined carcinomas, 35 were classified as m carcinomas including carcinoma in situ, whereas 45 were sm carcinomas, Fifteen (42.9%) of the 35 m and 30 (66.7%) of the 45 sm carcinomas demonstrated high vascular density, whereas 20 (57.1%) m and 15 (33.3%) sm carcinomas showed moderate or low vascular density, Vascular density was higher in sm carcinomas than in m carcinomas and there was a significant correlation between depth of invasion and vascular density. Of the 45 highly vascularized carcinomas, 44 expressed platelet-derived endothelial cell growth factor, There was a statistically significant correlation between the frequency of platelet-derived endothelial cell growth factor expression and microvessel density (P = 0.012). These data demonstrate that microvessel density may be associated with the depth of cancer invasion and that platelet-derived endothelial cell growth factor may play an important role in the early stage of colon cancer development through angiogenesis.