p600, a unique protein required for membrane morphogenesis and cell survival
p600, a unique protein required for membrane morphogenesis and cell survival
复制标题
DOI：
10.1073/pnas.0507458102
复制
发表时间：
2005-10-18
影响因子：
11.1
通讯作者：
Herlitz, AM
中科院分区：
文献类型：
--
作者：
Nakatani, Y;Konishi, H;Herlitz, AM
在这篇文章中，我们鉴定和表征了p600，一种独特600-kDa视网膜母细胞瘤蛋白和钙调素结合蛋白。在细胞核中，p600和视网膜母细胞瘤蛋白似乎充当染色质支架。在细胞质中，p600和网格蛋白形成网状结构，这可能有助于细胞骨架组织和膜形态发生。用干扰RNA降低p600的表达消除了整合素介导的皱褶膜形成，并且进一步防止了整合素介导的存活途径的激活。因此，p600的敲低使细胞对由细胞脱离诱导的细胞凋亡敏感。这些发现为肿瘤发生中近膜事件的调节提供了机制性的见解。
in this article, we identify and characterize p600, a unique 600-kDa retinoblastoma protein- and calmodulin-binding protein. In the nucleus, p600 and retinoblastoma protein seem to act as a chromatin scaffold. In the cytoplasm, p600 and clathrin form a meshwork structure, which could contribute to cytoskeletal organization and membrane morphogenesis. Reduced expression of p600 with interference RNA abrogates integrin-mediated ruffled membrane formation and, furthermore, prevents activation of integrin-mediated survival pathways. Consequently, knockdown of p600 sensitizes cells to apoptosis induced by cell detachment. These findings provide mechanistic insight into the regulation of membrane-proximal events in tumorigenesis.