Hepatitis B virus (HBV) particles are produced in a cell culture system by transient expression of transfected HBV DNA.
Hepatitis B virus (HBV) particles are produced in a cell culture system by transient expression of transfected HBV DNA.
复制标题
乙型肝炎病毒 (HBV) 颗粒是通过转染的 HBV DNA 瞬时表达在细胞培养系统中产生的。
DOI：
10.1073/pnas.84.9.2678
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发表时间：
1987
影响因子：
11.1
通讯作者：
K. Koike
中科院分区：
文献类型：
--
作者：
K. Yaginuma;Y. Shirakata;M. Kobayashi;K. Koike
以人肝癌细胞HuH-7为受体，通过瞬时表达转染的HBV DNA，建立了生产B型肝炎病毒（HBV）颗粒的体外系统。观察到的3.6-和2.2-脱氢酶转录本与病毒感染的肝细胞中的转录本相似。这两个转录本揭示了其5'端的微异质性。证明了RNA转录后病毒相关颗粒的形成。在细胞质中观察到的核心颗粒和在培养基中分泌的病毒颗粒含有HBV DNA的复制中间体，其条带密度分别为1.35-1.36 g/cm ~ 3和1.22-1.24 g/cm ~ 3。此外，模板HBV DNA的体外诱变表明，P基因以及C基因产物是产生HBV颗粒所必需的。
An in vitro system for the production of hepatitis B virus (HBV) particles was established by the transient expression of transfected HBV DNA using a human hepatocellular carcinoma cell, HuH-7, as a recipient. The 3.6- and 2.2-kilobase transcripts observed were similar to those in virus-infected liver cells. Both transcripts revealed the microheterogeneity of their 5' ends. The formation of virus-related particles subsequent to the RNA transcription was demonstrated. The core particles observed in the cytoplasm and the virus particles secreted in the culture medium contained the replicative intermediates of HBV DNA and banded at densities of 1.35-1.36 g/cm3 and 1.22-1.24 g/cm3, respectively. Furthermore, the in vitro mutagenesis of the template HBV DNA demonstrated that the P gene as well as the C gene products were essential for the production of HBV particles.