Cellular migration through the cardiac jelly matrix: a stereoanalysis by high-voltage electron microscopy.
Cellular migration through the cardiac jelly matrix: a stereoanalysis by high-voltage electron microscopy.
复制标题
细胞通过心脏果冻基质的迁移：高压电子显微镜的立体分析。
DOI：
10.1016/0012-1606(82)90178-6
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发表时间：
1982
影响因子：
2.7
通讯作者：
Markwald,RR
中科院分区：
文献类型：
--
作者：
Fitzharris,TP;Markwald,RR
用扫描电镜和高压电镜立体分析方法，对鸡心脏发育过程中垫组织的形成和迁移进行了研究。采用两种固定方法，提高细胞外基质（心胶）的水溶性成分的保存：1%单宁酸在3%戊二醛（TAG）和1%氯化十六烷基吡啶（CPC）在3%戊二醛。我们的研究结果表明，保存的细胞：基质的相互作用所表现出的内皮细胞和迁移垫组织是依赖于固定的方法。在TAG固定的胚胎，丝状伪足延伸从endoorthodial以及丝状伪足的先驱细胞是最经常与微纤维成分的矩阵，而在CPC固定的材料，这些相同的细胞延伸被发现与多形性的富含透明质酸盐的纤维束。在这些初始的细胞与基质的相互作用后，内分泌细胞和先驱细胞，拖尾细胞侵入细胞外基质区，并在两种固定类型中清楚地遇到不同的微环境，在其中参与细胞：基质协会。这些观察结果支持了这一假设，即丝状伪足探测endoorthodial细胞和先锋细胞的结果在大分子重排的矩阵，从而表明一个额外的功能丝状伪足超越易位的细胞。
Formation and migration of cushion tissue in the developing chick heart was analyzed by scanning and high-voltage electron microscopic stereoanalysis. Two methods of fixation which enhance the preservation of water-soluble components of the extracellular matrix (cardiac jelly) were employed: 1% tannic acid in 3% glutaraldehyde (TAG) and 1% cetylpyridinium chloride (CPC) in 3% glutaraldehyde. Our results indicated that the preservation of the cell: matrix interaction exhibited by endocardial cells and migrating cushion tissue is dependent upon the method of fixation. In TAG-fixed embryos, filopodial extensions from the endocardium as well as filopodia of pioneering cells are most often associated with microfibrillar components of the matrix, whereas in CPC-fixed material these same cellular extensions are found in association with pleomorphic anastomosing strands rich in hyaluronate. Following these initial cell:matrix interactions by both the endocardium and pioneering cells, trailing cells invade the extracellular matrical region and clearly encounter in both types of fixation a different microenvironment in which to engage in cell:matrical associations. These observations support the hypothesis that filopodial probing by endocardial cells and pioneering cells results in macromolecular reorderings of the matrix and thus suggest an additional function for filopodia beyond translocation of the cells.