Histones H3 and H4 require their relevant amino-tails for efficient nuclear import and replication-coupled chromatin assembly in vivo.
Histones H3 and H4 require their relevant amino-tails for efficient nuclear import and replication-coupled chromatin assembly in vivo.
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DOI：
10.1038/s41598-017-03218-6
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发表时间：
2017-06-08
影响因子：
4.6
通讯作者：
Thiriet C
中科院分区：
文献类型：
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作者：
Ejlassi A;Menil-Philippot V;Galvani A;Thiriet C
伴随的染色质组装和 DNA 复制对于细胞生存和基因组完整性至关重要，并且需要新合成的组蛋白。尽管新合成的 H3 和 H4 的 N 端结构域具有关键功能，但它们对复制偶联染色质组装的要求仍存在争议。利用绒泡菌中外源蛋白自发内化的独特能力，我们证明 H3 和 H4 N 尾在 S 期期间的核输入中具有关键功能，但对于组装成核小体来说不是必需的。然而，我们的数据表明，呈现异位 N 末端结构域的复合物 S 相中的染色质组装是通过复制独立机制发生的。我们发现复制依赖性染色质组装需要具有相关 N 尾结构域的 H3/H4 复合物，这表明组蛋白伴侣同时识别两个组蛋白结构域。
Concomitant chromatin assembly and DNA duplication is essential for cell survival and genome integrity, and requires newly synthesized histones. Although the N-terminal domains of newly synthesized H3 and H4 present critical functions, their requirement for replication-coupled chromatin assembly is controversial. Using the unique capability of the spontaneous internalization of exogenous proteins in Physarum, we showed that H3 and H4 N-tails present critical functions in nuclear import during the S-phase, but are dispensable for assembly into nucleosomes. However, our data revealed that chromatin assembly in the S-phase of complexes presenting ectopic N-terminal domains occurs by a replication-independent mechanism. We found that replication-dependent chromatin assembly requires an H3/H4 complex with the relevant N-tail domains, suggesting a concomitant recognition of the two histone domains by histone chaperones.