Characterization of an IL-6-like molecule from an echinoderm (Asterias forbesi)
Characterization of an IL-6-like molecule from an echinoderm (Asterias forbesi)
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DOI：
10.1006/cyto.1996.0069
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发表时间：
1996-07-01
期刊：
影响因子：
3.8
通讯作者：
Habicht, GS
中科院分区：
文献类型：
--
作者：
Beck, G;Habicht, GS
我们以前的研究已经证明无脊椎动物体内存在三种脊椎动物炎症细胞因子中的两种，即白介素1和肿瘤坏死因子。我们以棘皮动物体腔液为研究对象，确定其是否含有第三种炎性细胞因子IL-6。用凝胶筛层析和制备等电聚焦相结合的方法对体腔液进行浓缩和分离，观察到无脊椎动物IL-6样分子的M(R)约为30 000，等电点为5.5。用兔抗人IL-6多克隆血清进行Western印迹分析，在约30 000 Da处检测到一条带无脊椎动物IL-6在B9实验中被人IL-6抗血清抑制。最后，体腔细胞在脂多糖刺激后12h就能释放IL-6样分子，这些结果与我们的其他数据一起，表明棘皮动物具有所有三种脊椎动物炎症细胞因子的相关性。(C)1996年学术出版社有限公司
Our previous studies have demonstrated the presence of two of the three vertebrate inflammatory cytokines in invertebrates, namely, interleukin (IL)-1 and tumour necrosis factor (TNF), We studied the coelomic fluid of the echinoderm, Asterias forbesi, to determine whether it contained the third inflammatory cytokine, IL-6, Coelomic fluids were concentrated and then fractionated by a combination of gel sieve chromatography and preparative isoelectric focusing, The M(r) observed for the invertebrate IL-6-like molecule was approximate to 30 000 and the pI was 5.5, Western blot analysis using a polyclonal rabbit antiserum to human IL-6 detected a band at approximate to 30 000 Da, The invertebrate IL-6 was inhibited by an antiserum to human IL-6 when used in the B9 assay Finally, the coelomocytes were found to be capable of releasing the IL-6-like molecule as early as 12 h following stimulation by LPS, These results, together with our other data, show that echinoderms possess correlates of all three vertebrate inflammatory cytokines. (C) 1996 Academic Press Limited